First report of orchid fleck virus and its mite vector on green cordyline
| Autor: | Aline Daniele Tassi, Elliot W. Kitajima, Juliana Freitas-Astúa, Ralf G. Dietzgen |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine biology Nucleic acid sequence Plant Science Amplicon biology.organism_classification Cordyline 01 natural sciences Virology Nucleoprotein 03 medical and health sciences 030104 developmental biology Vector (epidemiology) Degenerate primer Mite Orchid fleck virus VETORES DE DOENÇAS DE PLANTAS Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | Orchid fleck virus (OFV) and its mite vector Brevipalpus californicus were for the first time identified on green cordyline plants showing distinctive chlorotic and necrotic ringspots. Thin section electron microscopy revealed bacilliform, dichorhavirus-like particles within nuclear viroplasms. RT-PCR using OFV degenerate primers yielded a single amplicon, the nucleotide sequence of which closely matched the nucleoprotein gene of OFV. |
| Databáze: | OpenAIRE |
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