In vitro and in vivo differentiation of induced pluripotent stem cells generated from urine-derived cells into cardiomyocytes
| Autor: | Yafeng Zhou, Qing Rui, Yufeng Jiang, Nan-Nan Zhang, Hua-Jia Yang, Min Chen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
QH301-705.5 Science Biology Noninvasive Regenerative medicine General Biochemistry Genetics and Molecular Biology Pathogenesis 03 medical and health sciences Transduction (genetics) 0302 clinical medicine In vivo Biology (General) Induced pluripotent stem cell Stem therapy Reprogramming In vitro Cell biology 030104 developmental biology Stem cell General Agricultural and Biological Sciences 030217 neurology & neurosurgery Research Article |
| Zdroj: | Biology Open, Vol 7, Iss 1 (2018) Biology Open |
| ISSN: | 2046-6390 |
| Popis: | Generation of human cardiomyocytes from cells derived from various sources, including skin biopsy, has been made possible by breakthrough advances in stem cell research. However, it is attractive to build up a negligibly invasive way to create induced pluripotent stem (iPS) cells. In this study, we created iPS cells from human urine-derived epithelial cells by gene transduction using lentiviral vectors in a totally noninvasive manner. Then, we induced the differentiation of iPS cells into functional cardiomyocytes both in vitro and in vivo. Action potentials were recorded in putative cardiomyocytes and spontaneous beating cells were observed. Our results offered an alternative method to generate cardiomyocytes in a totally noninvasive manner from an easily accessible source. The availability of urine and its potent reprogramming characteristics will provide opportunities for the use of cells with specific genotypes to study the pathogenesis and molecular mechanisms of disease in vitro. Summary: We generated and verified iPS cells from urine-derived cells in a totally noninvasive manner and induced the differentiation of iPS cells into functional cardiomyocytes both in vitro and in vivo. |
| Databáze: | OpenAIRE |
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