Conserved transcriptional regulation of a cone phototransduction gene in vertebrates
| Autor: | Andrea S. Viczian, Michael E. Zuber, M. Verardo, Debora B. Farber, Barry E. Knox |
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| Rok vydání: | 2004 |
| Předmět: |
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Transcription Genetic genetic structures Xenopus Biophysics Electrophoretic Mobility Shift Assay Biology Biochemistry Retina Green fluorescent protein Animals Genetically Modified Xenopus laevis Structural Biology Transcription (biology) Genetics Transcriptional regulation Animals Phosphodiesterase Molecular Biology Gene DNA Primers Regulation of gene expression Reporter gene Photoreceptor Base Sequence Cell Biology biology.organism_classification Immunohistochemistry Molecular biology Gene Expression Regulation Vertebrates sense organs Transcription Cone Signal Transduction Visual phototransduction |
| Zdroj: | FEBS Letters. 577:259-264 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/j.febslet.2004.10.008 |
| Popis: | cGMP-phosphodiesterase (PDE) is a key component in visual phototransduction. Rod and cone photoreceptors each produce their unique cGMP-PDE subunits. The alpha' catalytic subunits are believed to be cone-specific. In this study, we report that transfection of the -132 to +139 sequence in the upstream region of the human alpha'-PDE gene fused to luciferase cDNA gives the highest level of reporter gene transcription in cultured retinoblastoma Y79 cells. Transgenic Xenopus laevis carrying this sequence fused to green fluorescent protein (GFP) expressed GFP in cones, suggesting a conserved regulatory mechanism for alpha'-PDE transcription in both human and frog. |
| Databáze: | OpenAIRE |
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