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A study was conducted to evaluate the effects of plant spacing and N fertilizer application on dry matter yield and chemical composition of Urochloa hybrid cv. Mulato II grass for the first 150 days after planting. A factorial experiment with 3 urea fertilizer levels (0, 50 and 100 kg/ha) and 4 spacings between plants and rows (20 × 20, 30 × 40, 40 × 60 and 50 × 80 cm) with 3 replications was used. Data collected were dry matter yield (DMY), leaf:stem ratio and chemical analyses, i.e. crude protein (CP), ash, neutral detergent fiber (NDF), acid detergent fiber (ADF) and acid detergent lignin (ADL) concentrations. Results indicated that DMY, leaf:stem ratio, CP%, NDF% and ADF% were significantly (P |