MECHANISM OF TOLERANCE FOLLOWING CLASS I-DISPARATE RENAL ALLOGRAFTS IN MINIATURE SWINE
| Autor: | David H. Sachs, Philip C. Guzzetta, Takao Suzuki, Kaoru Sakamoto, Eric O. Kortz, Joan K. Lunney, Caroline H. Chester |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Interleukin 2
Time Factors Swine medicine.medical_treatment Miniature swine Biology Major histocompatibility complex Immune tolerance Immune Tolerance medicine Animals Immunity Cellular Transplantation Kidney Graft Survival Histocompatibility Antigens Class I Immunosuppression Kidney Transplantation In vitro Tolerance induction medicine.anatomical_structure Histocompatibility Immunology biology.protein Swine Miniature Lymphocyte Culture Test Mixed medicine.drug |
| Zdroj: | Transplantation. 49:1142-1149 |
| ISSN: | 0041-1337 |
| DOI: | 10.1097/00007890-199006000-00023 |
| Popis: | Previous studies utilizing a recombinant MHC haplo-type in our partially inbred miniature swine herd have demonstrated that some recipients matched only for SLA class II show long-term acceptance of renal allografts without exogenous immunosuppression. Such animals have been shown to develop systemic tolerance as evidenced by prolonged rejection times of subsequent donor-specific, but not third-party, skin grafts. In the present studies in vitro cellular responses of long-term tolerant animals and of 7 animals studied sequentially are presented. Long-term tolerant animals demonstrated responses consistent with the absence of the class I reactive helper populations normally present in naive controls. Animals studied sequentially segregated into two groups based on cellular reactivity and survival. All animals showed complete loss of antidonor class I cell-mediated lymphocytolytic (CML) reactivity by postoperative day 10. However, animals surviving less than 20 days maintained CML reactivity to donor class I plus third-party class II in the posttransplant period, while animals surviving greater than 40 days lost such reactivity. Addition of exogenous interleukin 2 could not reverse this loss. These studies suggest that tolerance induction to a renal allograft across a class I only difference involves effects on both helper and killer class I reactive cell populations. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|