Increased expression of Fcgamma receptors II and III on macrophages of rheumatoid arthritis patients results in higher production of tumor necrosis factor alpha and matrix metalloproteinase

Autor: Fons A. J. van de Loo, Pilar Barrera, Timothy R D J Radstake, Leo A. B. Joosten, Peter L E M van Lent, Arjen B. Blom, Wim B. van den Berg, Annet W. Sloetjes, Fred G. J. Sweep, A.E.M. Holthuysen, G.J. Pesman
Rok vydání: 2003
Předmět:
Male
Tissue engineering and reconstructive surgery [UMCN 4.3]
Cellular immunity
medicine.medical_treatment
Immunology
Arthritis
Inflammation
Enzyme-Linked Immunosorbent Assay
Monocytes
Proinflammatory cytokine
Arthritis
Rheumatoid
Immunoenzyme Techniques
Rheumatology
Immunology and Allergy
Medicine
Gelatinase
Humans
Pharmacology (medical)
RNA
Messenger
Cells
Cultured
Aged
DNA Primers
Chronic inflammation and autoimmunity [UMCN 4.2]
business.industry
Reverse Transcriptase Polymerase Chain Reaction
Tumor Necrosis Factor-alpha
Endocrinology and reproduction [UMCN 5.2]
Macrophages
Receptors
IgG
Synovial Membrane
Immunotherapy
gene therapy and transplantation [UMCN 1.4]
Middle Aged
medicine.disease
Flow Cytometry
Interleukin-12
medicine.anatomical_structure
Cytokine
Tumor necrosis factor alpha
Female
medicine.symptom
Synovial membrane
Matrix Metalloproteinase 1
business
Interleukin-1
Zdroj: Arthritis and Rheumatism, 48, 4, pp. 1002-14
Arthritis and Rheumatism, 48, 1002-14
ISSN: 0004-3591
Popis: Item does not contain fulltext OBJECTIVE: To evaluate Fcgamma receptor (FcgammaR) expression on synovial macrophages from rheumatoid arthritis (RA) patients and to determine whether this expression correlates with the production of the proinflammatory cytokines tumor necrosis factor alpha (TNFalpha), interleukin-1beta (IL-1beta), IL-12, and matrix metalloproteinase 1 (MMP-1). We also sought to determine whether mature macrophages from RA patients express aberrant levels of FcgammaRI, FcgammaRII, and FcgammaRIII, and to determine the production of inflammatory mediators after immune complex (IC) stimulation. METHODS: Immunohistochemistry was performed on cryostat sections of synovial biopsy specimens obtained from 27 RA patients and 5 controls. FcgammaR I, II, and III were detected, as well as the proinflammatory mediators IL-1, TNFalpha, IL-12, and MMP-1. Monocytes were isolated from the blood of 10 RA patients and 10 healthy controls and cultured for 7 days with macrophage colony-stimulating factor to obtain macrophages. Using fluorescence-activated cell sorting, the expression of FcgammaRI, FcgammaRII, and FcgammaRIII was determined. On day 7, macrophages were stimulated with heat-aggregated gamma globulins (HAGGs) for 24 hours. Production of cytokines was measured using enzyme-linked immunosorbent assay, and production of gelatinases/collagenases was measured by degradation of fluorescent gelatin. RESULTS: Immunohistochemistry showed higher FcgammaRII and FcgammaRIII expression in RA synovium than in controls. FcgammaRII and FcgammaRIII, but not FcgammaRI, were highly correlated with the number of synovial macrophages. Consistent with this, TNFalpha expression correlated positively with FcgammaRIII expression. Moreover, MMP-1 expression strongly correlated with FcgammaR I, II, and III expression. Mature macrophages from RA patients showed significantly enhanced expression of FcgammaRII and FcgammaRIII compared with controls. Twenty-four hours after stimulation of RA macrophages with HAGGs, significantly higher production of TNFalpha and gelatinase/collagenase was measured. CONCLUSION: RA synovium and mature RA macrophages express significantly elevated levels of FcgammaRII and FcgammaRIII, resulting in much higher production of TNFalpha and gelatinase/collagenase after IC stimulation. These data suggest that disturbed expression of FcgammaR on mature synovial macrophages is involved in the pathology of RA.
Databáze: OpenAIRE
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