Protective effect of quercetin in ecto-enzymes, cholinesterases, and myeloperoxidase activities in the lymphocytes of rats exposed to cadmium
| Autor: | Jamile F. Gonçalves, Roberta Schmatz, Pauline da Costa, Daniela Zanini, Cinthia M. Mazzanti, Fátima Husein Abdalla, Luciane Belmonte Pereira, Victor Camera Pimentel, Cibele Lima Lhamas, Maria Rosa Chitolina Schetinger, Andréia Machado Cardoso, Lizielle Souza de Oliveira, Jucimara Baldissarelli, Vera Maria Morsch, Caroline Curry Martins |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Antioxidant Adenosine Deaminase Aché medicine.medical_treatment Clinical Biochemistry Pharmacology Protective Agents chemistry.chemical_compound Adenosine deaminase Toxicity Tests medicine Animals Cholinesterases heterocyclic compounds Lymphocytes Pyrophosphatases Rats Wistar Molecular Biology Butyrylcholinesterase Peroxidase Dose-Response Relationship Drug biology Hydrolysis Cell Biology General Medicine Adenosine language.human_language Biochemistry chemistry Myeloperoxidase Acetylcholinesterase biology.protein language Quercetin Ex vivo Cadmium medicine.drug |
| Zdroj: | Molecular and Cellular Biochemistry. 396:201-211 |
| ISSN: | 1573-4919 0300-8177 |
| DOI: | 10.1007/s11010-014-2155-7 |
| Popis: | The ex vivo and in vitro effects of quercetin on NTPDase, adenosine deaminase (ADA), and acetycholinesterase (AChE) activities in lymphocytes, as well as the effects of quercetin on butyrylcholinesterase (BChE) activity in serum and myeloperoxidase (MPO) activity in plasma were determined in rats. For the ex vivo experiment, animals were orally exposed to Cadmium (Cd) for 45 days. Animals were divided into eight groups: saline/ethanol, saline/Querc 5 mg/kg, saline/Querc 25 mg/kg, saline/Querc 50 mg/kg, Cd/ethanol, Cd/Querc 5 mg/kg, Cd/Querc 25 mg/kg, and Cd/Querc 50 mg/kg. The ex vivo data showed an increase in the ATP and ADP hydrolysis and ADA activity in Cd-exposed rats when compared to the control group. The treatment with quercetin 25 and 50 mg/kg prevented this increase in the ATP and ADP hydrolysis, while the treatment with quercetin 5, 25, and 50 mg/kg prevented the increase in the ADA activity. AChE, BChE, and MPO activities ex vivo presented an increase in the Cd-exposed group when compared to the control group, and the treatment with quercetin 5, 25, and 50 mg/kg prevented this increase caused by Cd exposure. The in vitro experiment showed that quercetin 5, 10, 25, or 50 µM decreased the ADA activity proportionally to the increase of the concentrations of quercetin when compared to the control group. Thus, we can suggest that the quercetin is able to modulate NTPDase, ADA, AChE, and MPO activities and contribute to maintain the levels of ATP, adenosine, and acetylcholine normal, respectively, exhibiting potent pro-inflammatory and anti-inflammatory actions. |
| Databáze: | OpenAIRE |
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