Evaluation of vascular tone and cardiac contractility in response to silver nanoparticles, using Langendorff rat heart preparation
| Autor: | Carmen G. Gonzalez, Pedro Pablo Martinez-Cuevas, Hector Rosas-Hernandez, Cuauhtémoc Oros-Ovalle, Alejandro Ramirez-Lee Manuel, Mariela Bravo-Sanchez, Gabriel Alejandro Martínez-Castañón |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Silver Biomedical Engineering Metal Nanoparticles Nitric Oxide Synthase Type II Pharmaceutical Science Medicine (miscellaneous) Bioengineering 02 engineering and technology In Vitro Techniques Pharmacology Nitric Oxide medicine.disease_cause Nitric oxide Contractility 03 medical and health sciences chemistry.chemical_compound medicine Animals General Materials Science Rats Wistar Phenylephrine Chemistry Cardiac muscle Heart 021001 nanoscience & nanotechnology Rats Cardiovascular physiology Oxidative Stress 030104 developmental biology medicine.anatomical_structure Biochemistry Vasoconstriction cardiovascular system Molecular Medicine medicine.symptom 0210 nano-technology Perfusion Oxidative stress Muscle Contraction medicine.drug |
| Zdroj: | Nanomedicine: Nanotechnology, Biology and Medicine. 13:1507-1518 |
| ISSN: | 1549-9634 |
| DOI: | 10.1016/j.nano.2017.01.017 |
| Popis: | Silver nanoparticles (AgNPs) have been widely used because of their antimicrobial properties. However, several reports suggest that AgNPs exposure promote cardiac effects that involve nitric oxide (NO) and oxidative stress (OS). Nevertheless, there are no studies related to AgNPs-induced effects in cardiac physiology. The aim of this study was to evaluate the AgNPs direct actions on coronary vascular tone and cardiac contractility using Langendorff rat heart preparation. Low concentrations of AgNPs (0.1 and 1 μg/mL) increased NO derived from inducible NO-synthase (iNOS), without modifying cardiac parameters. Meanwhile, high concentrations (10 and 100 μg/mL) promoted a sustained vasoconstriction and increased cardiac contractility related to OS, leading to rhabdomyolysis. Furthermore, AgNPs were internalized in the cardiac muscle, hindering classic actions induced by phenylephrine (Phe) and acetylcholine (ACh). These data suggest that AgNPs affect cardiac physiology in function of the concentration and in part of the NO generation, NOS expression and OS. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect