Toll‐like receptors 2 and 3 enhance melanogenesis and melanosome transport in human melanocytes
| Autor: | Ryoko Shimada-Ohmori, Saaya Koike, Kenichiro Tsuchiyama, Mai Inoue, Setsuya Aiba, Takeshi Yamauchi, Kenshi Yamasaki |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Lipopolysaccharides
0301 basic medicine Tyrosinase chemical and pharmacologic phenomena Dermatology General Biochemistry Genetics and Molecular Biology Melanin 03 medical and health sciences 0302 clinical medicine Humans Cells Cultured Melanosome Melanins Toll-like receptor Melanosomes integumentary system Chemistry Biological Transport Listeria monocytogenes Toll-Like Receptor 2 Toll-Like Receptor 3 Cell biology TLR2 Poly I-C 030104 developmental biology Epidermal Cells Oncology Melanosome transport 030220 oncology & carcinogenesis TLR3 Melanocytes Dopachrome tautomerase |
| Zdroj: | Pigment Cell & Melanoma Research. 31:570-584 |
| ISSN: | 1755-148X 1755-1471 |
| DOI: | 10.1111/pcmr.12703 |
| Popis: | Because little is known about how the innate immune response influences skin pigmentation, we examined whether Toll-like receptor (TLR) agonists participate in melanogenesis and melanosome transportation. We observed that TLR2/2 agonist HKLM and TLR3 agonist Poly(I:C) increased the amount of extracellular melanin from primary human epidermal melanocytes. HKLM, but not Poly(I:C), increased the melanogenic genes such as tyrosinase and dopachrome tautomerase. Poly(I:C) increased the expression of Rab27A, a molecule that facilitates melanosome transport to perimembranous actin filament. UVB irradiation induced Rab27A and melanosome transportation in a similar manner of Poly(I:C). SiRNA for TLR3 or Rab27A suppressed the perimembranous accumulation of Gp100-positive vesicles in melanocytes and decreased melanin transfer to neighboring keratinocytes induced by both Poly(I:C) and UVB. These results suggest that the microenvironment in the epidermis and innate immune stimuli, such as microbiome and ultraviolet represented here by TLR2 and TLR3 agonists, could affect the melanogenesis in human melanocytes. |
| Databáze: | OpenAIRE |
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