Maslinic Acid, a Triterpene from Olive, Affects the Antioxidant and Mitochondrial Status of B16F10 Melanoma Cells Grown under Stressful Conditions
| Autor: | Leticia García-Salguero, Celeny Figuera, Juan Peragón, Eva E. Rufino-Palomares, Fernando J. Reyes-Zurita, José A. Lupiáñez, Pedro P. Medina, Khalida Mokhtari, Amalia Pérez-Jiménez |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
chemistry.chemical_classification
Reactive oxygen species Antioxidant Article Subject biology medicine.medical_treatment Reactive oxygen species (ROS) Olive lcsh:Other systems of medicine Glutathione lcsh:RZ201-999 Melanoma cancer Superoxide dismutase chemistry.chemical_compound Complementary and alternative medicine Biochemistry chemistry Catalase Maslinic acid biology.protein medicine Maslinic acid (MA) Viability assay Fetal bovine serum Research Article |
| Zdroj: | Digibug. Repositorio Institucional de la Universidad de Granada instname Evidence-based Complementary and Alternative Medicine : eCAM Evidence-Based Complementary and Alternative Medicine, Vol 2015 (2015) |
| Popis: | Maslinic acid (MA) is a natural compound whose structure corresponds to a pentacyclic triterpene. It is abundant in the cuticular lipid layer of olives. MA has many biological and therapeutic properties related to health, including antitumor, anti-inflammatory, antimicrobial, antiparasitic, antihypertensive, and antioxidant activities. However, no studies have been performed to understand the molecular mechanism induced by this compound in melanoma cancer. The objective of this study was to examine the effect of MA in melanoma (B16F10) cells grown in the presence or absence of fetal bovine serum (FBS). We performed cell proliferation measurements, and the reactive oxygen species (ROS) measurements using dihydrorhodamine 123 (DHR 123) and activities of catalase, glucose 6-phosphate dehydrogenase, glutathione S-transferase, and superoxide dismutase. These changes were corroborated by expression assays. FBS absence reduced cell viability decreasing IC50 values of MA.The DHR 123 data showed an increase in the ROS level in the absence of FBS. Furthermore, MA had an antioxidant effect at lower assayed levels measured as DHR and antioxidant defense.However, at higher dosagesMAinduced cellular damage by apoptosis as seen in the results obtained. This study has been supported, in part, by funds of the consolidated Research Group BIO-157, from the General Secretariat of Universities, Research and Technology of the Ministry of Economy, Innovation, Science and Employment Government of the Junta de Andaluc´ıa (Spain), and by the Research Contract no. C-3650-00 under the program FEDER-INNTERCONECTA from the Spanish Government and European Union FEDER funds. Amalia P´erez-Jim´enez is a recipient of a postdoctoral research fellowship Torres- Quevedo no. PTQ 12-05739. |
| Databáze: | OpenAIRE |
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