Identification and nucleotide sequence of the Leptospira biflexa serovar patoc trpE and trpG genes
| Autor: | David B. Yelton, S L Peng |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Genetics
Leptospira biology Base Sequence Genetic Complementation Test Molecular Sequence Data Restriction Mapping Nucleic acid sequence Tryptophan Microbiology Conserved sequence Open reading frame Genes Bacterial Codon usage bias biology.protein Anthranilate synthase Amino Acid Sequence ORFS Codon Peptide Chain Initiation Translational Molecular Biology Gene Peptide sequence Anthranilate Synthase Research Article |
| Zdroj: | Journal of bacteriology. 171(4) |
| ISSN: | 0021-9193 |
| Popis: | Leptospira biflexa is a representative of an evolutionarily distinct group of eubacteria. In order to better understand the genetic organization and gene regulatory mechanisms of this species, we have chosen to study the genes required for tryptophan biosynthesis in this bacterium. The nucleotide sequence of the region of the L. biflexa serovar patoc chromosome encoding the trpE and trpG genes has been determined. Four open reading frames (ORFs) were identified in this region, but only three ORFs were translated into proteins when the cloned genes were introduced into Escherichia coli. Analysis of the predicted amino acid sequences of the proteins encoded by the ORFs allowed us to identify the trpE and trpG genes of L. biflexa. Enzyme assays confirmed the identity of these two ORFs. Anthranilate synthase from L. biflexa was found to be subject to feedback inhibition by tryptophan. Codon usage analysis showed that there was a bias in L. biflexa towards the use of codons rich in A and T, as would be expected from its G + C content of 37%. Comparison of the amino acid sequences of the trpE gene product and the trpG gene product with corresponding gene products from other bacteria showed regions of highly conserved sequence. |
| Databáze: | OpenAIRE |
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