Assessing pathogenic mutations in dental follicles as an attempt to identify early events in odontogenic tumours tumourigenesis
| Autor: | Bruna Pizziolo Coura, Vanessa Fátima Bernardes, Taynara Asevedo Campos de Resende, Vinicius Cesar Barbosa De Menezes, Marina Gonçalves Diniz, Carolina Cavaliéri Gomes, Sílvia Ferreira de Sousa, Ricardo Santiago Gomez |
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| Rok vydání: | 2020 |
| Předmět: |
Adenoma
Proto-Oncogene Proteins B-raf 0301 basic medicine Pathology medicine.medical_specialty Carcinogenesis Odontogenic Tumors medicine.disease_cause Proto-Oncogene Proteins p21(ras) 03 medical and health sciences symbols.namesake 0302 clinical medicine stomatognathic system Humans Medicine Ameloblastoma neoplasms General Dentistry Anterior teeth Sanger sequencing Dental follicle Mutation business.industry Dental Sac 030206 dentistry Cell Biology General Medicine medicine.disease digestive system diseases Odontogenic stomatognathic diseases 030104 developmental biology Otorhinolaryngology symbols KRAS business Mandibular molar |
| Zdroj: | Archives of Oral Biology. 113:104523 |
| ISSN: | 0003-9969 |
| Popis: | Objective Driver oncogenic mutations have been reported in several benign neoplasms. While ameloblastomas show BRAF p.V600E mutations, adenomatoid odontogenic tumours harbour either KRAS p.G12R or p.G12 V. The lack of understanding of the core molecular changes involved in tumour initiation and progression represents a critical barrier to developing new strategies for cancer detection and prevention. Considering the fact that ameloblastoma and adenomatoid odontogenic tumours can originate from dental follicles, we hypothesized that the BRAF and KRAS mutations might be early events in odontogenic tumours tumourigenesis. We aimed to assess BRAF and KRAS mutations in dental follicles associated with asymptomatic impacted teeth. Design Forty-eight dental follicles containing odontogenic epithelial remnants were included in the study. As ameloblastomas most often occur in the posterior mandible and adenomatoid odontogenic tumours have a predilection for the anterior jaws, we assessed by allele-specific qPCR the presence of BRAF p.V600E in 32 dental follicles associated with impacted 3rd mandibular molar teeth and KRAS p.G12 V and KRAS p.G12R mutations in 16 dental follicle specimens obtained from around impacted anterior teeth. Sanger sequencing was used as an additional method. Results None of the dental follicle cases tested positive for the mutations. Conclusion In conclusion, we tried to detect the early genetic events associated with odontogenic tumours development in dental follicles, but we were unable to showcase that BRAF p.V600E and KRAS p.G12R or p.G12 V mutations are the early genetic events associated with odontogenic tumours development. |
| Databáze: | OpenAIRE |
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