Lectin binding of human sperm associates with DEFB126 mutation and serves as a potential biomarker for subfertility
| Autor: | Xiaoxi Sun, Yangyang Sun, Shiwei Duan, Yonglian Zhang, Huijuan Shi, Sheng-Ce Tao, Bin Wu, Yan-Cheng Wu, Peng Wang, Yuan Yao, Hua Diao, Shumin Zhou, Changgen Shi, Li Cheng, Yihua Gu, Guowu Chen, Ju-fen Zheng, Aijie Xin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Glycan Glycosylation beta-Defensins Genotype Gene Expression Article Male infertility 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Polysaccharides Lectins medicine Humans Amino Acid Sequence Sperm motility Infertility Male 030219 obstetrics & reproductive medicine Multidisciplinary biology Glycobiology urogenital system Lectin medicine.disease Sperm Spermatozoa 030104 developmental biology Biochemistry chemistry Immunology Mutation biology.protein Jacalin Biomarkers Protein Binding |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Coating on the sperm surface, glycocalyx, plays a key role in sperm motility, maturation and fertilization. A comprehensive profile of sperm surface glycans will greatly facilitate both basic researches and clinical studies. Because of the capability of recognizing different glycan moieties, lectins are widely used in glycobiology. However, lacking high-throughput technology, limited lectins have been reported for analyzing the glycan of human sperm. In this study, we employed a lectin microarray for profiling the surface glycans of human sperm, on which 54 out of 91 lectins showed positive binding. Based on this technique, we compared lectin binding profiling of sperm with homozygous DEFB126 mutation (del/del) with that of wild type (wt/wt). DEFB126 was reported to contribute to the sialylation on sperm surface and its homozygous mutation was related to male subfertility. Six lectins (Jacalin/AIA, GHA, ACL, MPL, VVL and ABA) were found to develop lower binding affinity to sperm with del/del. Further validation showed that these lectins, especially ABA and MPL, can be potential biomarkers for clinical diagnosis of subfertility due to the mutation of DEFB126. Our research provides insight into the detection of some unexplained male subfertility and the lectin microarray is generally applicable for infertility/subfertility sperm biomarker discovery. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|