ScPNP-A, a plant natriuretic peptide from Stellera chamaejasme, confers multiple stress tolerances in Arabidopsis
| Autor: | Tianshu Wang, Na Fan, Youfeng Yang, Yahui Wei, Huirui Guan, Yanping Fu, Dian Meng, Jia-kun Dai, Wei He, Xin Liu, Bin Guo |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology Regulator Arabidopsis Gene Expression Plant Science Biology 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Biosynthesis Stress Physiological Genetics Extracellular Natriuretic Peptides Gene Abiotic stress fungi food and beverages Biotic stress biology.organism_classification Plants Genetically Modified Cell biology Droughts 030104 developmental biology chemistry Thymelaeaceae Plant Stomata Signal transduction 010606 plant biology & botany |
| Zdroj: | Plant physiology and biochemistry : PPB. 149 |
| ISSN: | 1873-2690 |
| Popis: | As a class of peptide hormone, plant natriuretic peptides (PNPs) play an important role in maintaining water and salt balance in plants, as well as in the physiological processes of biotic stress and pathogen resistance. However, in plants, except for some PNPs, such as the Arabidopsis thaliana PNP-A (AtPNP-A), of which the function has not yet been thoroughly revealed, few PNPs in other plants have been reported. In this study, a PNP-A (ScPNP-A) has been identified and characterized in Stellera chamaejasme for the first time. ScPNP-A is a double-psi beta-barrel (DPBB) fold containing protein and is localized in the extracellular (secreted) space. In S. chamaejasme, the expression of ScPNP-A was significantly up-regulated by salt, drought and cold stress. Changes at the physiological and biochemical levels and the expression of resistance-related genes indicated that overexpression of ScPNP-A can significantly improve salt, drought and freezing tolerance in Arabidopsis. ScPNP-A could stimulate the opening, not the closing of stomata, and its expression was not enhanced by external application of ABA. Furthermore, overexpression of ScPNP-A resulted in the elevated expression of genes in the ABA biosynthesis and reception pathway. These suggested that there may be some cross-talk between ScPNP-A and the ABA-dependent signaling pathways to regulate water related stress, however further experimentation is required to understand this relationship. In addition, overexpression of ScPNP-A can enhance the resistance to pathogens by enhancing SAR in Arabidopsis. These results indicate that ScPNP-A could function as a positive regulator in plant response to biotic stress and abiotic stress. |
| Databáze: | OpenAIRE |
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