Retinoblastoma Gene Product-Associated Proteins in Human Colon Cancer Cell Lines
| Autor: | J. P. Harvey, Ahktar Afshan Ali, D. M. Wildrick, B. M. Boman |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Immunoprecipitation
Radioimmunoassay Biophysics Adenocarcinoma Biology medicine.disease_cause Biochemistry Cell Line Gene product Cyclin D1 Tumor Cells Cultured medicine Humans Genes Retinoblastoma Lung Molecular Biology Gene Carcinoma Transitional Cell Retinoblastoma Antibodies Monoclonal Cancer Cell Biology medicine.disease Molecular biology eye diseases Molecular Weight Urinary Bladder Neoplasms Cell culture Colonic Neoplasms Electrophoresis Polyacrylamide Gel Carrier Proteins Retinoblastoma-Binding Protein 2 Carcinogenesis |
| Zdroj: | Biochemical and Biophysical Research Communications. 194:848-854 |
| ISSN: | 0006-291X |
| Popis: | The expression of cellular proteins that associate with the human retinoblastoma (RB1) gene protein was examined in three colorectal carcinoma cell lines (SW480, SW403, DiFi) by immunoprecipitation analysis, using the mouse monoclonal antibody (RB-MAb-1) directed against the RB1 gene product (RB). The potential of RB-MAb-1 to detect RB-associated proteins by immunoprecipitation analysis was confirmed using three control cell lines (WI-38, J82, T24) known to express RB and/or RB-associated proteins. In all colon cancer cell lines tested, multiple RB-associated protein (RAP) bands were found with molecular weights of 30-50 kDa. Because involvement of the RB1 gene in colorectal tumorigenesis appears to differ from its role in other cancer types (wherein RB1 is lost or inactivated), it will be important to characterize the role of RAPs in RB growth regulatory mechanisms of colonic epithelial cells. |
| Databáze: | OpenAIRE |
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