Methylmercury-induced cytotoxicity and oxidative biochemistry impairment in dental pulp stem cells: the first toxicological findings

Autor: Roberta Souza D'Almeida Couto, Bruna Puty, Priscila Cunha Nascimento, Edivaldo Herculano Corrêa de Oliveira, Carlos Augusto Galvão Barboza, Lygia Sega Nogueira, Rafael Rodrigues Lima, Laís Bonfim, Renata Duarte de Souza-Rodrigues, Márcia Martins Marques, Leonardo Oliveira Bittencourt
Rok vydání: 2021
Předmět:
Zdroj: PeerJ, Vol 9, p e11114 (2021)
PeerJ
Repositório Digital do Instituto Evandro Chagas (Patuá)
Instituto Evandro Chagas (IEC)
instacron:IEC
ISSN: 2167-8359
DOI: 10.7717/peerj.11114
Popis: Federal University of Par?. Institute of Arts Sciences. Bel?m, PA, Brazil / University of Par?. Institute of Biological Sciences. Laboratory of Functional and Structural Biology. Bel?m, PA, Brazil. University of Par?. Institute of Biological Sciences. Laboratory of Functional and Structural Biology. Bel?m, PA, Brazil / Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Laborat?rio de Cultura de Tecidos e Citogen?tica. Ananindeua, PA, Brasil. University of Par?. Institute of Biological Sciences. Laboratory of Functional and Structural Biology. Bel?m, PA, Brazil / Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Laborat?rio de Cultura de Tecidos e Citogen?tica. Ananindeua, PA, Brasil. University of Par?. Institute of Biological Sciences. Laboratory of Functional and Structural Biology. Bel?m, PA, Brazil / Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Laborat?rio de Cultura de Tecidos e Citogen?tica. Ananindeua, PA, Brasil.. University of Par?. Institute of Biological Sciences. Laboratory of Functional and Structural Biology. Bel?m, PA, Brazil. University of Par?. Institute of Biological Sciences. Laboratory of Functional and Structural Biology. Bel?m, PA, Brazil. Federal University of Par?. Faculty of Dentistry. Bel?m, PA, Brazil. Federal University of Rio Grande do Norte. Department of Morphology. Natal, RN, Brazil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Laborat?rio de Cultura de Tecidos e Citogen?tica. Ananindeua, PA, Brasil. Ibirapuera University. School of Dentistry. Graduation Program. S?o Paulo, SP, Brazil. University of Par?. Institute of Biological Sciences. Laboratory of Functional and Structural Biology. Bel?m, PA, Brazil. Background: Methylmercury (MeHg) is a potent toxicant able to harm human health, and its main route of contamination is associated with the consumption of contaminated fish and other seafood. Moreover, dental amalgams are also associated with mercury release on human saliva and may contribute to the accumulation of systemic mercury. In this way, the oral cavity seems to be the primary location of exposure during MeHg contaminated food ingestion and dental procedures but there is a lack of literature about its effects on dental tissues and the impact of this toxicity on human health. In this way, this study aimed to analyze the effects of different doses of MeHg on human dental pulp stem cells after short-term exposure. Methods: Dental pulp stem cells from human exfoliated deciduous teeth (SHED) were treated with 0.1, 2.5 and 5 ?M of MeHg during 24 h. The MeHg effects were assessed by evaluating cell viability with Trypan blue exclusion assay. The metabolic viability was indirectly assessed by MTT reduction assay. In order to evaluate an indicative of antioxidant defense impairment, cells exposed to 0.1 and 5 ?M MeHg were tested by measuring glutathione (GSH) level. Results: It was observed that cell viability decreased significantly after exposure to 2.5 and 5 ?M of MeHg, but the metabolic viability only decreased significantly at 5 ?M MeHg exposure, accompanied by a significant decrease in GSH levels. These results suggest that an acute exposure of MeHg in concentrations higher than 2.5 ?M has cytotoxic effects and reduction of antioxidant capacity on dental pulp stem cells.
Databáze: OpenAIRE
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