Nitric oxide overcomes Cd and Cu toxicity in in vitro-grown tobacco plants through increasing contents and activities of rubisco and rubisco activase

Autor: Alaaldin Idris H. Khairy, Mi Jeong Oh, Seung Min Lee, Da Som Kim, Kwang Soo Roh
Rok vydání: 2016
Předmět:
inorganic chemicals
Chlorophyll
0106 biological sciences
0301 basic medicine
NaCl
Sodium chloride
Research paper
PGK
Phosphoglycerate kinase
Rubisco
NADH
Nicotinamide adenine dinucleotide (reduced form)
NaHCO3
Sodium bicarbonate (Sodium hydrogen carbonate)
Rubisco activase
MgCl2
Magnesium chloride
DTT
Dithiothreitol
NO
Nitric oxide
01 natural sciences
Biochemistry
lcsh:Biochemistry
SNP
Sodium nitroprusside
03 medical and health sciences
PEG 10K
Polyethylene glycol 10
000
Rubisco
Ribulose 1
5bisphosphate carboxylase/oxygenase
Cu
Copper
PVPP
Polyvinylpolypyrrolidone
lcsh:QD415-436
Sodium nitroprusside
GSH
Glutathione
KCl
Potassium chloride
MBT
Mercaptabemzathiazol
RuBP
Ribulose 1
5-bisphosphate
DMF
N
N-Dimethylformamide
fungi
food and beverages
KHCO3
Potassium bicarbonate
Nitric oxide
EDTA
Ethylenediaminetetraacetic acid
PK
Pyruvate kinase
BTP
Bis tris phosphate
030104 developmental biology
PMSF
Phenylmethanesulfonyl fluoride
ATP
Adenosine triphosphate
Cd
Cadmium
(NH4)2SO4
Ammonium sulphate
PEP
Phosphoenolpyruvate
Cadmium
010606 plant biology & botany
Zdroj: Biochimie Open
Biochimie Open, Vol 2, Iss C, Pp 41-51 (2016)
ISSN: 2214-0085
DOI: 10.1016/j.biopen.2016.02.002
Popis: Toxic heavy metals such as cadmium (Cd) and copper (Cu) are global problems that are a growing threat to the environment. Despite some heavy metals are required for plant growth and development, others are considered toxic elements and do not play any known physiological role in plant cells. Elevated doses of Cd or Cu cause toxicity in plants and generate damages due to the stress condition and eventually cause a significant reduction in quantity and quality of crop plants. The nitric oxide (NO) donor sodium nitroprusside (SNP) is reported to alleviate the toxicity of some heavy metals like Cd and Cu. In the current study, the role of NO in alleviating stresses of Cd and Cu was investigated in in vitro-grown tobacco (Nicotiana tabacum) Based on plant growth, total chlorophyll contents, contents and activities of rubisco and rubisco activase. According to the results of this study, the growth and total chlorophyll contents of Cd/Cu stressed plants were hugely decreased in the absence of SNP, while the supplementation of SNP resulted in a significant increase of both fresh weight and total chlorophyll contents. Remarkable reductions of Rubisco and rubisco activase contents and activities were observed in Cd and Cu-induced plants. SNP supplementation showed the highest contents and activities of rubisco and rubisco activase compared to the control and Cu/Cd-stressed plants. Taken together, our findings suggest that SNP could play a protective role in regulation of plant responses to abiotic stresses such as Cd and Cu by enhancing Rubisco and Rubisco activase.
Graphical abstract
Highlights • Heavy metal ions are believed to act as growth inhibitors and environmental disruptors. • Nitric oxide (NO) plays a functional role in regulation of plant responses to abiotic stresses. • Rubisco involved in the process of atmospheric carbon fixation in photosynthesis. • Rubisco Catalyzes 2 types of reactions (carboxylation and oxygenation). • Rubisco activase removes bound RuBP from inactive, decarboxylated Rubisco in an ATP-dependent reaction.
Databáze: OpenAIRE
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