Myocardial ultrastructure in systole and diastole using ballistic cryofixation
| Autor: | R. Grier Monroe, Walter J. Gamble, S. Bullivant, C. G. La Farge, A. Rosenthal, R. Gamboa, C. L. Morgan |
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| Rok vydání: | 1968 |
| Předmět: |
Materials science
Explosive material Biopsy Diastole Cryofixation law.invention Myocardial ultrastructure law Heart Rate Formaldehyde Freezing Methods Humans Supersonic speed Molecular Biology Projectile Muscles Myocardium Blood Pressure Determination Anatomy Coolant Microscopy Electron Spirometry Electron microscope Biomedical engineering Muscle Contraction |
| Zdroj: | Journal of ultrastructure research. 22(1) |
| ISSN: | 0022-5320 |
| Popis: | A method is described whereby sections of myocardium were obtained for electron microscopy from an actively beating heart during specific phases of its contraction cycle. Small section were rapidly removed by hollow projectiles accelerated to supersonic velocities by an explosive. Cryofixation was achieved using a mixture of liquid and frozen propane as the coolant. The method employed is discussed in terms of its application, results, and limitations. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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