Two-dimensional electrophoretic display of restriction fragments from genomic DNA
| Autor: | Andrew C. Peacock, Sylvia L. Bunting, Michael M. Seidman, Susan P.C. Cole |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Biophysics
Deoxyribonuclease HindIII Biochemistry Restriction fragment Deoxyribonuclease EcoRI chemistry.chemical_compound Restriction map Animals Molecular Biology Electrophoresis Agar Gel biology Deoxyribonuclease BamHI Cell Biology DNA DNA Restriction Enzymes Molecular biology Rats Restriction enzyme genomic DNA chemistry Genes biology.protein Agarose Amplified fragment length polymorphism Restriction digest Restriction fragment length polymorphism |
| Zdroj: | Analytical biochemistry. 149(1) |
| ISSN: | 0003-2697 |
| Popis: | We have developed a procedure for the resolution of restriction enzyme digests of mammalian genomic DNA in two dimensions. Fragments from a first digestion are separated on a column of purified agarose containing a second restriction enzyme in the absence of the divalent cation required for enzyme activity. After enzyme activation and digestion, the fragments are resolved on an agarose slab gel. We have digested rat genomic DNA and found in the ethidium-stained pattern a variety of features which have not been described previously. |
| Databáze: | OpenAIRE |
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