Hydrogen photoproduction in green algae Chlamydomonas reinhardtii sustainable over 2 weeks with the original cell culture without supply of fresh cells nor exchange of the whole culture medium
| Autor: | Takumi Isono, Daisuke Momose, Shigeru Mineki, Kyohei Yamashita, Takafumi Yagi, Norihide Okada, Eiji Tokunaga |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Time Factors Light Hydrogen Cell Culture Techniques chemistry.chemical_element Chlamydomonas reinhardtii 02 engineering and technology Plant Science 03 medical and health sciences Botany Hydrogen production biology Spectrum Analysis Plant physiology 021001 nanoscience & nanotechnology Pulp and paper industry biology.organism_classification Culture Media 030104 developmental biology chemistry Cell culture Green algae Desiccator 0210 nano-technology Anaerobic exercise Sulfur |
| Zdroj: | Journal of Plant Research. 129:771-779 |
| ISSN: | 1618-0860 0918-9440 |
| Popis: | Unicellular green algae Chlamydomonas reinhardtii are known to make hydrogen photoproduction under the anaerobic condition with water molecules as the hydrogen source. Since the hydrogen photoproduction occurs for a cell to circumvent crisis of its survival, it is only temporary. It is a challenge to realize persistent hydrogen production because the cells must withstand stressful conditions to survive with alternation of generations in the cell culture. In this paper, we have found a simple and cost-effective method to sustain the hydrogen production over 14 days in the original culture, without supply of fresh cells nor exchange of the culture medium. This is achieved for the cells under hydrogen production in a sulfur-deprived culture solution on the {anaerobic, intense light} condition in a desiccator, by periodically providing a short period of the recovery time (2 h) with a small amount of TAP(+S) supplied outside of the desiccator. As this operation is repeated, the response time of transition into hydrogen production (preparation time) is shortened and the rate of hydrogen production (build up time) is increased. The optimum states of these properties favorable to the hydrogen production are attained in a few days and stably sustained for more than 10 days. Since generations are alternated during this consecutive hydrogen production experiment, it is suggested that the improved hydrogen production properties are inherited to next generations without genetic mutation. The properties are reset only when the cells are placed on the {sulfur-sufficient, aerobic, moderate light} conditions for a long time (more than 1 day at least). |
| Databáze: | OpenAIRE |
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