Satellite Cells Say NO to Radiation
| Autor: | Charles L. Limoli, Michael J. Baker, Jennie Cho-Lim, Vincent J. Caiozzo, Erich Giedzinski, Bertrand P. Tseng |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Nitroprusside Satellite Cells Skeletal Muscle metabolism [Myogenic Regulatory Factors] pharmacology [Imidazoles] Biophysics Cell Count pharmacology [Nitroprusside] Biology Nitric Oxide MyoD Article Cyclic N-Oxides Rats Sprague-Dawley Medicine and Health Sciences Animals SNP Radiology Nuclear Medicine and imaging Myogenin Cell Proliferation Radiation pharmacology [Cyclic N-Oxides] Cell growth Imidazoles drug effects radiation effects [Cell Differentiation] Cell Differentiation Dose-Response Relationship Radiation Free Radical Scavengers biology.organism_classification Biomechanical Phenomena Rats Cell biology Dose–response relationship pharmacology [Free Radical Scavengers] biosynthesis metabolism [Nitric Oxide] Myogenic Regulatory Factors Gamma Rays Immunology Myogenic regulatory factors Satellite (biology) Stem cell radiation effects [Cell Proliferation] cytology metabolism radiation effects [Satellite Cells Skeletal Muscle] |
| Zdroj: | Cho-Lim, Jennie J; Caiozzo, Vincent J; Tseng, Bertrand P; Giedzinski, Erich; Baker, Mike J; & Limoli, Charles L. (2011). Satellite cells say NO to radiation.. Radiation research, 175(5), 561-568. UC Irvine: Institute for Clinical and Translational Science. Retrieved from: http://www.escholarship.org/uc/item/3nm4t3v4 |
| ISSN: | 1938-5404 0033-7587 |
| DOI: | 10.1667/rr2453.1 |
| Popis: | Skeletal muscles are commonly exposed to radiation for diagnostic procedures and the treatment of cancers and heterotopic bone formation. Few studies have considered the impact of clinical doses of radiation on the ability of satellite cells (myogenic stem cells) to proliferate, differentiate and contribute to recovering/maintaining muscle mass. The primary objective of this study was to determine whether the proliferation of irradiated satellite cells could be rescued by manipulating NO levels via pharmacological approaches and mechanical stretch (which is known to increase NO levels). We used both SNP (NO donor) and PTIO (NO scavenger) to manipulate NO levels in satellite cells. We observed that SNP was highly effective in rescuing the proliferation of irradiated satellite cells, especially at doses less than 5 Gy. The potential importance of NO was further illustrated by the effects of PTIO, which completely inhibited the rescue effect of SNP. Mechanical cyclic stretch was found to produce significant increases in NO levels of irradiated satellite cells, and this was associated with a robust increase in satellite cell proliferation. The effects of both radiation and NO on two key myogenic regulatory factors (MyoD and myogenin) were also explored. Irradiation of satellite cells produced a significant increase in both MyoD and myogenin, effects that were mitigated by manipulating NO levels via SNP. Given the central role of myogenic regulatory factors in the proliferation and differentiation of satellite cells, the findings of the current study underscore the need to more fully understand the relationship between radiation, NO and the functionality of satellite cells. |
| Databáze: | OpenAIRE |
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