Non-specific interactions of antibody-oligonucleotide conjugates with living cells
| Autor: | Marc Nothisen, Alain Wagner, Isabelle Kuhn, Sergii Kolodych, Sarah Cianférani, Victor Lehot, Stéphane Erb, Guilhem Chaubet |
|---|---|
| Přispěvatelé: | Conception et application de molécules bioactives (CAMB), Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Département Sciences Analytiques et Interactions Ioniques et Biomoléculaires (DSA-IPHC), Institut Pluridisciplinaire Hubert Curien (IPHC), Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Syndivia SAS |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
[SDV]Life Sciences [q-bio] Cell Oligonucleotides Medicinal chemistry 02 engineering and technology Non specific Drug safety Multidisciplinary biology Drug discovery Chemistry 021001 nanoscience & nanotechnology Chemical biology 3. Good health Nucleic acids medicine.anatomical_structure Biochemistry Medicine Antibody 0210 nano-technology hal-03200308 Cell Survival Science Biologics Sciences du Vivant [q-bio]/Biochimie Biologie Moléculaire Article Antibodies 03 medical and health sciences Cell Line Tumor medicine Humans Nucleic-acid therapeutics Pharmacology Oligonucleotide Ligand binding assay Proteins DNA Trastuzumab Cell selectivity Kinetics 030104 developmental biology Drug delivery Nucleic acid biology.protein Antibody therapy Chemical modification Conjugate |
| Zdroj: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-9 (2021) Scientific Reports, Nature Publishing Group, 2021, 11 (1), ⟨10.1038/s41598-021-85352-w⟩ |
| ISSN: | 2045-2322 |
| Popis: | Antibody-Oligonucleotide Conjugates (AOCs) represent an emerging class of functionalized antibodies that have already been used in a wide variety of applications. While the impact of dye and drug conjugation on antibodies’ ability to bind their target has been extensively studied, little is known about the effect caused by the conjugation of hydrophilic and charged payloads such as oligonucleotides on the functions of an antibody. Previous observations of non-specific interactions of nucleic acids with untargeted cells prompted us to further investigate their impact on AOC binding abilities and cell selectivity. We synthesized a series of single- and double-stranded AOCs, as well as a human serum albumin-oligonucleotide conjugate, and studied their interactions with both targeted and non-targeted living cells using a time-resolved analysis of ligand binding assay. Our results indicate that conjugation of single strand oligonucleotides to proteins induce consistent non-specific interactions with cell surfaces while double strand oligonucleotides have little or no effect, depending on the preparation method. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink