Immunostimulatory CpG-Oligodeoxynucleotides Cause Extramedullary Murine Hemopoiesis
| Autor: | T, Sparwasser, L, Hültner, E S, Koch, A, Luz, G B, Lipford, H, Wagner |
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| Rok vydání: | 1999 |
| Předmět: |
Mice
Inbred BALB C Mice Inbred C3H Immunology Radiation-Protective Agents Mice SCID Hematopoietic Stem Cells Colony-Forming Units Assay Mice Inbred C57BL Mice Adjuvants Immunologic Oligodeoxyribonucleotides Bone Marrow Hematopoiesis Extramedullary Radiation Chimera Splenomegaly Mice Inbred CBA Animals Immunology and Allergy CpG Islands Female |
| Zdroj: | The Journal of Immunology. 162:2368-2374 |
| ISSN: | 1550-6606 0022-1767 |
| Popis: | Bacterial DNA and the synthetic CpG-oligodeoxynucleotides (ODNs) derived thereof have attracted attention because they activate cells of the adaptive immune system (lymphocytes) and the innate immune system (APCs) in a sequence-dependent manner. Here, we addressed whether CpG-ODNs affect hemopoiesis. Challenging mice with immunostimulatory CpG-ODN sequences led to transient splenomegaly, with a maximum increase of spleen weight at day 6. The induction of splenomegaly by CpG-ODNs was sequence-specific, dose-dependent, and associated with an increase in splenic cell count, in numbers of granulocyte-macrophage CFUs (GM-CFUs), and early erythroid progenitors (burst-forming units-erythroid). The transfer of spleen cells from CpG-ODN-pretreated animals into lethally irradiated syngeneic mice yielded an increase of spleen CFUs. Furthermore, the challenge of sublethally irradiated mice with CpG-ODNs caused radioprotective effects, in that recovery of GM-CFUs and cytotoxic T cell function was enhanced. The increase in GM-CFU and CTL function correlated with an enhanced resistance to Listeria infection in irradiated mice. We conclude from these data that CpG-ODNs trigger extramedullary hemopoiesis, and that this finding could be of therapeutic relevance in myelosuppression. |
| Databáze: | OpenAIRE |
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