Circulating tumor cells mirror bone metastatic phenotype in prostate cancer
| Autor: | Andreas Josefsson, Karin Welén, Jan-Erik Damber, Helena Brisby, Karin Larsson, Marianne Månsson, Jens Björkman, Daniel Åhs, Eva Rohlova |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
business.industry Metastatic phenotype circulating tumor cells medicine.disease Primary tumor skeletal metastases of prostate cancer 03 medical and health sciences Prostate cancer liquid biopsies 030104 developmental biology 0302 clinical medicine Circulating tumor cell Oncology 030220 oncology & carcinogenesis Urologi och njurmedicin medicine Cancer research Urology and Nephrology business Research Paper |
| Zdroj: | Oncotarget |
| Popis: | Circulating tumor cells (CTCs) are promising biomarkers in prostate cancer (PC) because they derive from primary tumor and metastatic tissues. In this study, we used quantitative real-time PCR (qPCR) to compare the expression profiles of 41 PC-related genes between paired CTC and spinal column metastasis samples from 22 PC patients that underwent surgery for spinal cord compression. We observed good concordance between the gene expression profiles in the CTC and metastasis samples in most of the PC patients. Expression of nine genes (AGR2, AKR1C3, AR, CDH1, FOLH1, HER2, KRT19, MDK, and SPINK1) showed a significant correlation between the CTC and metastasis samples. Hierarchical clustering analysis showed a similar grouping of PC patients based on the expression of these nine genes in both CTC and metastasis samples. Our findings demonstrate that CTCs mirror gene expression patterns in tissue metastasis samples from PC patients. Although low detection frequency of certain genes is a limitation in CTCs, our results indicate the potential for CTC phenotyping as a tool to improve individualized therapy in metastatic prostate cancer. |
| Databáze: | OpenAIRE |
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