Differential evolution of antigenic regions of porcine reproductive and respiratory syndrome virus 1 before and after vaccine introduction
| Autor: | Dong-Uk Lee, Sang H. Je, Sung J. Yoo, Jun Woo Park, Young S. Lyoo, Choi-Kyu Park, Sun Young Sunwoo, Myung-Hyee Kim, Taeyong Kwon |
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| Rok vydání: | 2019 |
| Předmět: |
Cancer Research
Swine viruses animal diseases Porcine Reproductive and Respiratory Syndrome Sequence Homology Biology Evolution Molecular 03 medical and health sciences Phylogenetics Virology Animals Porcine respiratory and reproductive syndrome virus Selection Genetic Clade Phylogeny 030304 developmental biology 0303 health sciences Korea Attenuated vaccine Phylogenetic tree 030306 microbiology Genetic heterogeneity Structural gene Genetic Variation virus diseases Viral Vaccines respiratory system Porcine reproductive and respiratory syndrome virus biology.organism_classification Vaccination Infectious Diseases RNA Viral |
| Zdroj: | Virus Research. 260:12-19 |
| ISSN: | 0168-1702 |
| DOI: | 10.1016/j.virusres.2018.11.004 |
| Popis: | Porcine reproductive and respiratory syndrome virus (PRRSV) is a widespread viral pathogen that has caused tremendous economic losses throughout most pig-producing countries. Nowadays, both PRRSV-1 and PRRSV-2 co-circulate in Korean pig populations, and commercial modified live vaccine (MLV) is predominantly used to control PRRS. Specifically, control strategy using only PRRSV-2 MLV that was used since 1995 cannot prevent the spread of PRRSV-1 and damage from its infection, which led to the first introduction of two additional PRRSV-1 vaccines in 2014. Despite the wide implementation with PRRSV-1 vaccines, there is a lack of knowledge about the currently circulating Korean PRRSV-1 strains. Whole structural genes of PRRSV-1 before (11) and after (17) the introduction of vaccine were compared to determine the genetic evolutionary features of PRRSV. Genetic and phylogenetic analysis indicated that Korean PRRSV-1 shared 91.5 ± 1.7% nucleotide identity but formed a unique clade based on ORF2–7 phylogeny. Bioinformatics showed increased genetic heterogeneity, enhanced diversifying selection, and the emergence of novel glycosylation sites within neutralizing epitopes of minor structural proteins after vaccine introduction. Taken together, our data provide novel insight into the evolution of minor structural proteins of PRRSV-1 in the vaccination era. |
| Databáze: | OpenAIRE |
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