Semienclosed Tube Cultures of Bean Plants ( Phaseolus vulgaris L.) for Enumeration of Rhizobium phaseoli by the Most-Probable-Number Technique
| Autor: | Charles Yokoyama, Ricardo Silva Araujo, Jaime Maya-Flores, Fredrick A. Bliss, Frank B. Dazzo, Deborah Barnes-McConnell |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
education.field_of_study
Rhizobiaceae Ecology Inoculation Host (biology) Population food and beverages biochemical phenomena metabolism and nutrition Biology biology.organism_classification Applied Microbiology and Biotechnology Most probable number Botany Phaseolus Microorganism-Plant Interactions education Microbial inoculant Bacteria Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 52:954-956 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.52.4.954-956.1986 |
| Popis: | The semienclosed tube culture technique of Gibson was modified to permit growth of common bean ( Phaseolus vulgaris L.) roots in humid air, enabling enumeration of the homologous (nodule forming) symbiont, Rhizobium phaseoli , by the most-probable-number plant infection method. A bean genotype with improved nodulation characteristics was used as the plant host. This method of enumeration was accurate when tubes were scored 3 weeks after inoculation with several R. phaseoli strains diluted from aqueous suspensions, peat-based inoculants, or soil. A comparison of population sizes obtained by most-probable-number tube cultures and plate counts indicated that 1 to 3 viable cells of R. phaseoli were a sufficient inoculant to induce nodule formation. |
| Databáze: | OpenAIRE |
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