Specificity of interleukin–1β-induced changes in monoamine concentrations in hypothalamic nuclei: blockade by interleukin-1 receptor antagonist
| Autor: | P.S. Mohankumar, Sheba M.J. MohanKumar, S. K. Quadri |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Male
Biogenic Amines medicine.medical_specialty Hypothalamus Biology Rats Sprague-Dawley chemistry.chemical_compound Dopamine Internal medicine Electrochemistry medicine Animals Neurotransmitter Chromatography High Pressure Liquid Analysis of Variance General Neuroscience Arcuate Nucleus of Hypothalamus Median Eminence Rats Endocrinology Interleukin 1 receptor antagonist Monoamine neurotransmitter nervous system chemistry Median eminence Catecholamine Serotonin Interleukin-1 Paraventricular Hypothalamic Nucleus medicine.drug |
| Zdroj: | Brain Research Bulletin. 47:29-34 |
| ISSN: | 0361-9230 |
| DOI: | 10.1016/s0361-9230(98)00037-9 |
| Popis: | The purpose of this study was to examine specificity in the effects of interleukin-1beta (IL-1beta) on monoamines in various areas of the hypothalamus. Adult male rats were injected i.p. with saline or 2.5 or 5.0 microg of IL-1beta or were pretreated with 500 microg of IL-1 receptor antagonist (IL-1ra) followed 5 min later by 5 microg of IL-1beta. The paraventricular nucleus (PVN), arcuate nucleus (AN), median eminence (ME), and medial preoptic area (MPA) were microdissected and analyzed for neurotransmitter concentrations by high-performance liquid chromatography with electrochemical detection (HPLC-EC). In the PVN, IL treatment produced significant increases in the concentrations of norepinephrine (NE), dopamine (DA), DA metabolite dihydroxyphenylacetic acid (DOPAC), serotonin (5-HT), and its metabolite 5-hydroxyindoleacetic acid (5-HIAA). IL-1 treatment increased the concentrations of NE and DA in the AN but only of NE in the ME, and it was without any effect in the MPA. Pretreatment with IL-1ra completely blocked the IL-1 effects. It is concluded that IL-1 induces highly specific changes in monoamine metabolism in the hypothalamus, and the nature of these changes depends on specific hypothalamic nuclei. |
| Databáze: | OpenAIRE |
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