Molecular cloning and structural modelling of gamma-phospholipase A2 inhibitors from Bothrops atrox and Micrurus lemniscatus snakes
| Autor: | Rafael J. Borges, Juliana C. Sobrinho, Rodrigo G. Stábeli, Luiz S. Ozaki, Rudson J. Holanda, Carina G. Picelli, Carla F. C. Fernandes, Fabio M. Matioli, Marcos R.M. Fontes, Anderson M. Kayano, Leonardo A. Calderon, Andreimar M. Soares, Carlos A.H. Fernandes |
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| Přispěvatelé: | UNIR, Universidade Estadual Paulista (Unesp), Virginia Commonwealth University, PVE-CNPq, UNISL |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Phospholipase A2 inhibitor Antivenom Phospholipase Biology Molecular cloning complex mixtures Biochemistry Gamma type phospholipase A2 inhibitors Micrurus lemniscatus 03 medical and health sciences Structural Biology Bothrops Fosfolipases A2 Molecular Biology Gene Genetics Bothrops atrox 030102 biochemistry & molecular biology RNA Snakes General Medicine Anatomy biology.organism_classification Inhibitors of phospholipases A2 Phospholipases A2 030104 developmental biology |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Repositório Institucional da Universidade Federal do Ceará (UFC) Universidade Federal do Ceará (UFC) instacron:UFC |
| ISSN: | 0141-8130 |
| Popis: | Made available in DSpace on 2018-12-11T16:47:25Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-10-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Phospholipases A2 inhibitors (PLIs) produced by venomous and non-venomous snakes play essential role in this resistance. These endogenous inhibitors may be classified by their fold in PLIα, PLIβ and PLIγ. Phospholipases A2 (PLA2s) develop myonecrosis in snake envenomation, a consequence that is not efficiently neutralized by antivenom treatment. This work aimed to identify and characterize two PLIs from Amazonian snake species, Bothrops atrox and Micrurus lemniscatus. Liver tissues RNA of specimens from each species were isolated and amplified by RT-PCR using PCR primers based on known PLIγ gene sequences, followed by cloning and sequencing of amplified fragments. Sequence similarity studies showed elevated identity with inhibitor PLIγ gene sequences from other snake species. Molecular models of translated inhibitors' gene sequences resemble canonical three finger fold from PLIγ and support the hypothesis that the decapeptide (residues 107–116) may be responsible for PLA2 inhibition. Structural studies and action mechanism of these PLIs may provide necessary information to evaluate their potential as antivenom or as complement of the current ophidian accident treatment. Centro de Estudos de Biomoléculas Aplicadas a Saúde CEBio Fundação Oswaldo Cruz FIOCRUZ Fiocruz Rondônia and Departamento de Medicina Universidade Federal de Rondônia UNIR Departamento de Física e Biofísica Instituto de Biociências Universidade Estadual Paulista UNESP Center for the Study of Biological Complexity Life Sciences Virginia Commonwealth University PVE-CNPq Centro Universitário São Lucas UNISL Departamento de Física e Biofísica Instituto de Biociências Universidade Estadual Paulista UNESP |
| Databáze: | OpenAIRE |
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