Long-lasting In vivo Gene Silencing by Electrotransfer of shRNA Expressing Plasmid
| Autor: | Laetitia Hellaudais, Muriel Golzio, Jean-Michel Escoffre, Daniel Drocourt, Gérard Tiraby, Arnaud Debin, Jean-Paul Reynes, Justin Teissié |
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| Přispěvatelé: | Institut de pharmacologie et de biologie structurale (IPBS), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), InvivoGen Europe, Escoffre, Jean-Michel, Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Cancer Research
[SDV.BIO]Life Sciences [q-bio]/Biotechnology [SDV.BA] Life Sciences [q-bio]/Animal biology [SDV]Life Sciences [q-bio] Gene electrotransfer Biology Transfection Green fluorescent protein Small hairpin RNA Mice 03 medical and health sciences 0302 clinical medicine In vivo RNA interference Animals Humans Gene silencing Gene Silencing RNA Small Interfering Muscle Skeletal Gene ComputingMilieux_MISCELLANEOUS 030304 developmental biology Mice Inbred BALB C 0303 health sciences [SDV.BA]Life Sciences [q-bio]/Animal biology Genetic transfer Molecular biology [SDV.BIO] Life Sciences [q-bio]/Biotechnology [SDV] Life Sciences [q-bio] Electroporation Oncology 030220 oncology & carcinogenesis Female Plasmids |
| Zdroj: | Technology in Cancer Research and Treatment Technology in Cancer Research and Treatment, 2008, 7 (2), pp.109-116. ⟨10.1177/153303460800700203⟩ Technology in Cancer Research and Treatment, Adenine Press, 2008, 7 (2), pp.109-116. ⟨10.1177/153303460800700203⟩ |
| ISSN: | 1533-0346 1533-0338 |
| DOI: | 10.1177/153303460800700203⟩ |
| Popis: | RNA interference appears as a promising tool for therapeutic gene silencing. A key limit is the delivery of the siRNA. A safe approach is to use a physical method such as in vivo electropulsation with contact electrodes. Getting a long lived silencing can be better approached by using the in situ expression of shRNA. This is presently obtained by using co-electrotransfer of specific plasmids coding for expression and silencing of a fluorescent protein. Using a non invasive fluorescence imaging assay, electrodelivery in mouse muscles is observed to induce complete silencing over more than two months in a specific way. The proper choices of the plasmids (sequence and relative amounts) and of the electric pulsing conditions appear as key parameters in the successful silencing. |
| Databáze: | OpenAIRE |
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