Direct imaging of the circular chromosome in a live bacterium
| Autor: | Cees Dekker, Xuan Zheng, Jakub Wiktor, Fabai Wu, Aleksandre Japaridze, Jacob W. J. Kerssemakers |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
DNA Replication
DNA Bacterial 0301 basic medicine Intravital Microscopy Chromosomal Proteins Non-Histone Science General Physics and Astronomy 02 engineering and technology Bacterial genome size Computational biology Origin of replication Genome Article Chromosomes General Biochemistry Genetics and Molecular Biology Computational biophysics 03 medical and health sciences chemistry.chemical_compound Transcription (biology) Escherichia coli lcsh:Science Physics Multidisciplinary Bacteria Escherichia coli Proteins Circular bacterial chromosome DNA replication Chromosome General Chemistry Chromosomes Bacterial 021001 nanoscience & nanotechnology 3. Good health 030104 developmental biology Microscopy Fluorescence chemistry Nucleic Acid Conformation lcsh:Q DNA Circular Single-Cell Analysis 0210 nano-technology Genome Bacterial DNA |
| Zdroj: | Nature Communications, Vol 10, Iss 1, Pp 1-9 (2019) Nature Communications Nature Communications, 10(1) |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-019-10221-0 |
| Popis: | Although the physical properties of chromosomes, including their morphology, mechanics, and dynamics are crucial for their biological function, many basic questions remain unresolved. Here we directly image the circular chromosome in live E. coli with a broadened cell shape. We find that it exhibits a torus topology with, on average, a lower-density origin of replication and an ultrathin flexible string of DNA at the terminus of replication. At the single-cell level, the torus is strikingly heterogeneous, with blob-like Mbp-size domains that undergo major dynamic rearrangements, splitting and merging at a minute timescale. Our data show a domain organization underlying the chromosome structure of E. coli, where MatP proteins induce site-specific persistent domain boundaries at Ori/Ter, while transcription regulators HU and Fis induce weaker transient domain boundaries throughout the genome. These findings provide an architectural basis for the understanding of the dynamic spatial organization of bacterial genomes in live cells. Bacterial chromosomes are tightly packed, limiting structural analysis by imaging techniques. Here, by quantitative time-lapse single-cell imaging of widened Escherichia coli cells, Wu and Japaridze et al. show that the chromosome exhibits a ring-like torus topology and a dynamic domain structure. |
| Databáze: | OpenAIRE |
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