In Cellulo Bioorthogonal Catalysis by Encapsulated AuPd Nanoalloys: Overcoming Intracellular Deactivation
| Autor: | Belén Rubio-Ruiz, Ana M. Pérez-López, Laura Uson, M. Carmen Ortega-Liebana, Teresa Valero, Manuel Arruebo, Jose L. Hueso, Victor Sebastian, Jesus Santamaria, Asier Unciti-Broceta |
|---|---|
| Přispěvatelé: | Instituto de Salud Carlos III, European Commission |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Rubio Ruiz, B, Perez-Lopez, A, Uson, L, Ortega Liebana, M C, Valero, T, Arruebo, M, Hueso, J L, Sebastian, V, Santamaría, J & Unciti-Broceta, A 2023, ' In Cellulo Bioorthogonal Catalysis by Encapsulated AuPd Nanoalloys. Overcoming Intracellular Deactivation ', Nano Letters, vol. 23, no. 3, pp. 804-811 . https://doi.org/10.1021/acs.nanolett.2c03593 |
| ISSN: | 1530-6992 1530-6984 |
| Popis: | Bioorthogonal metallocatalysis has opened up a xenobiotic route to perform nonenzymatic catalytic transformations in living settings. Despite their promising features, most metals are deactivated inside cells by a myriad of reactive biomolecules, including biogenic thiols, thereby limiting the catalytic functioning of these abiotic reagents. Here we report the development of cytocompatible alloyed AuPd nanoparticles with the capacity to elicit bioorthogonal depropargylations with high efficiency in biological media. We also show that the intracellular catalytic performance of these nanoalloys is significantly enhanced by protecting them following two different encapsulation methods. Encapsulation in mesoporous silica nanorods resulted in augmented catalyst reactivity, whereas the use of a biodegradable PLGA matrix increased nanoalloy delivery across the cell membrane. The functional potential of encapsulated AuPd was demonstrated by releasing the potent chemotherapy drug paclitaxel inside cancer cells. Nanoalloy encapsulation provides a novel methodology to develop nanoreactors capable of mediating new-to-life reactions in cells. UK Research & Innovation (UKRI) Engineering & Physical Sciences Research Council (EPSRC) EP/N021134/1 ERC (Advanced Grant CADENCE) ERC-2016-ADG-742684 European Commission European Commission Joint Research Centre H2020-MSCA-IF-2014-658833 H2020-MSCA-IF-2018-841990 H2020-MSCA-IF-2016-749299 H2020-MSCA-IF-2019-895664 Spanish Government RTI2018-099019-A-I00 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|