Phytochemical analysis and antioxidant activity of the seed of Telfairia occidentalis Hook (Cucurbitaceae)
| Autor: | Olorunfemi A. Eseyin, Johnson Ekarika, Akaninyene Daniel, Essien Grace Emmanuel, Akpan Ukeme, Thomas S. Paul, Abdul Sattar Munavvar Zubaid, Ahmad Ashfaq, Sheryar Afzal, Emmanuel E. Attih |
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| Rok vydání: | 2017 |
| Předmět: |
Antioxidant
Chromatography Gas Magnetic Resonance Spectroscopy DPPH medicine.medical_treatment Butanols Phytochemicals Oleic Acids Plant Science 01 natural sciences Biochemistry Antioxidants Analytical Chemistry Nitric oxide chemistry.chemical_compound food Phenols Spectroscopy Fourier Transform Infrared medicine Hexanes Dichloromethane Traditional medicine 010405 organic chemistry Chemistry Organic Chemistry food.food 0104 chemical sciences Telfairia occidentalis 010404 medicinal & biomolecular chemistry Cucurbitaceae Phytochemical Seeds Methanol Stearic Acids Oleic Acid |
| Zdroj: | Natural product research. 32(4) |
| ISSN: | 1478-6427 |
| Popis: | The 2,2-diphenyl-1-picryl hydrazyl (DPPH) radical, nitric oxide, reducing power, hydrogen peroxide scavenging, and total antioxidant activities of the methanol extract, n-hexane, dichloromethane, ethyl acetate, butanol and aqueous fractions of the seed of Telfairia occidentalis were evaluated. Total phenolic content was determined using the Folin-Ciocalteu method. The dichloromethane fraction exhibited the highest DPPH radical scavenging, reducing power and total antioxidant activities. Two pure compounds which were identified by FTIR, H-and 2D NMR and Mass spectroscopy as 9-octadecenoic acid (TOS B) and 10-hydroxyoctadecanoic acid (TOS C) and four oily isolates, TOS A, TOS D, TOS E and TOS F were obtained from the dichloromethane fraction. TOS E had the highest DPPH radical scavening activity comparable to that of ascorbic acid. GC-MS analysis revealed the major compounds in TOS E as 4-(2,2-Dimethyl-6-methylene cyclohexylidene)-2-butanol; 3-(3-hydroxybutyl)-2,4,4-trimethyl-2-cyclohexene-1-one and 1,2-Benzenedicarboxylic acid disooctyl ester. Thus, the seed of T. occidentalis can be consumed for its antioxidant property. |
| Databáze: | OpenAIRE |
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