Evaluation of laser induced sarcomere micro-damage: Role of damage extent and location in cardiomyocytes

Autor: Thorben Klamt, Stefan Kalies, Dominik Müller, Alexander Heisterkamp, Lara Gentemann
Rok vydání: 2021
Předmět:
Force generation
Muscle Physiology
Physiology
Sarcomere
law.invention
Rats
Sprague-Dawley
Mathematical and Statistical Techniques
Myofibrils
law
Animal Cells
Image Processing
Computer-Assisted
Medicine and Health Sciences
Myocyte
Myocytes
Cardiac
Dewey Decimal Classification::500 | Naturwissenschaften
Cells
Cultured
Cardiomyocytes
Multidisciplinary
Fourier Analysis
Total Cell Counting
Cell Differentiation
Optical Equipment
Femtosecond
Physical Sciences
Medicine
Engineering and Technology
Cellular Types
Anatomy
Algorithms
Research Article
Muscle Contraction
Statistical Distributions
Sarcomere alignment
Sarcomeres
Cell Physiology
Micro damage
Science
Cell Enumeration Techniques
Muscle Tissue
Equipment
Biology
Research and Analysis Methods
Animals
Muscle Cells
Lasers
Biology and Life Sciences
Cell Biology
Laser
Probability Theory
Myocardial Contraction
Rats
Cell Metabolism
Biological Tissue
Biophysics
Dewey Decimal Classification::600 | Technik::610 | Medizin
Gesundheit
Entire cell
Mathematics
Zdroj: PLoS ONE
PLoS ONE, Vol 16, Iss 6, p e0252346 (2021)
ISSN: 1932-6203
Popis: Whereas it is evident that a well aligned and regular sarcomeric structure in cardiomyocytes is vital for heart function, considerably less is known about the contribution of individual elements to the mechanics of the entire cell. For instance, it is unclear whether altered Z-disc elements are the reason or the outcome of related cardiomyopathies. Therefore, it is crucial to gain more insight into this cellular organization. This study utilizes femtosecond laser-based nanosurgery to better understand sarcomeres and their repair upon damage. We investigated the influence of the extent and the location of the Z-disc damage. A single, three, five or ten Z-disc ablations were performed in neonatal rat cardiomyocytes. We employed image-based analysis using a self-written software together with different already published algorithms. We observed that cardiomyocyte survival associated with the damage extent, but not with the cell area or the total number of Z-discs per cell. The cell survival is independent of the damage position and can be compensated. However, the sarcomere alignment/orientation is changing over time after ablation. The contraction time is also independent of the extent of damage for the tested parameters. Additionally, we observed shortening rates between 6–7% of the initial sarcomere length in laser treated cardiomyocytes. This rate is an important indicator for force generation in myocytes. In conclusion, femtosecond laser-based nanosurgery together with image-based sarcomere tracking is a powerful tool to better understand the Z-disc complex and its force propagation function and role in cellular mechanisms.
Databáze: OpenAIRE
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