DNA sequence analysis of a small cryptic plasmid from Lactococcus lactis subsp. lactis M14
| Autor: | N. R. S. Varma, Son Radu, Abdul Rahim Raha, Khatijah Yusoff, W Y Hooi, N.S. Mariana |
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| Rok vydání: | 2005 |
| Předmět: |
Sequence analysis
Base pair Operon Molecular Sequence Data DNA Single-Stranded Open Reading Frames Plasmid Sequence Homology Nucleic Acid Lactococcus Amino Acid Sequence Cloning Molecular Molecular Biology Genetics biology Base Sequence COPG Lactococcus lactis Nucleic acid sequence DNA Restriction Enzymes Sequence Analysis DNA biology.organism_classification Molecular biology Blotting Southern Rolling circle replication Plasmids |
| Zdroj: | Plasmid. 56(1) |
| ISSN: | 0147-619X |
| Popis: | A small plasmid designated pAR141 was isolated from Lactococcus lactis subsp. lactis M14 and its complete 1,594 base pair nucleotide sequence was determined. Analysis of the sequence indicated that this plasmid does not carry any industrially important determinants besides the elements involved in plasmid replication and control. The transcriptional repressor CopG and replication initiation protein RepB appeared as a single operon. A small countertranscribed RNA (ctRNA) coding region was found between the copG and repB genes. The double strand origin (dso) and single strand origin (sso) of rolling circle replicating (RCR) plasmids were also identified in pAR141, suggesting that this plasmid replicates by rolling circle (RC) mode. This observation was supported by S1 nuclease and Southern hybridization analyses. |
| Databáze: | OpenAIRE |
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