Argadin, a New Chitinase Inhibitor, Produced by Clonostachys sp. FO-7314
| Autor: | Satoshi Omura, Rokuro Masuma, Noriko Arai, Yuuichi Yamaguchi, Kazuro Shiomi, Andreas Turberg, Heinz Kölbl, Yuzuru Iwai |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Stereochemistry
Size-exclusion chromatography Peptides Cyclic Pentapeptide repeat Drug Discovery Animals Enzyme Inhibitors Mycelium chemistry.chemical_classification Molecular Structure biology Strain (chemistry) Chemistry Spectrum Analysis Chitinases General Chemistry General Medicine biology.organism_classification Cyclic peptide Microscopy Electron Enzyme Biochemistry Lucilia cuprina Chitinase biology.protein Mitosporic Fungi |
| Zdroj: | Chemical and Pharmaceutical Bulletin. 48:1442-1446 |
| ISSN: | 1347-5223 0009-2363 |
| DOI: | 10.1248/cpb.48.1442 |
| Popis: | A new chitinase inhibitor, designated as argadin (1), was isolated from the cultured broth of a fungal strain FO-7314. The strain was identified as Clonostachys sp. from the morphological characteristics. Argadin was purified from the cultured mycelium by a combination of cation exchange, adsorption and gel filtration chromatographic methods. The structure of argadin was elucidated as cyclo(Nomega-acetyl-L-arginyl-D-prolyl-homoseryl-histidyl-L- 2-aminoadipyl) in which homoseryl gamma-methylene bonded to histidyl alpha-amino residue. The IC50 value of argadin against Lucilia cuprina (blowfly) chitinase was 150 nM at 37 degrees C and 3.4 nM at 20 degrees C. Argadin arrested the moult of cockroach larvae upon injection into the ventral abdominal part. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|