cAMP response element-binding protein 1 feedback loop is necessary for consolidation of long-term synaptic facilitation in Aplysia
| Autor: | Shreyansh Shah, Diasinou Fioravante, Rong Yu Liu, John H. Byrne |
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| Rok vydání: | 2008 |
| Předmět: |
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Physiological biology General Neuroscience Response element Long-Term Potentiation Neural facilitation Nerve Tissue Proteins biology.organism_classification CREB Coculture Techniques Structural Homology Protein Aplysia Synaptic plasticity Synapses biology.protein Animals Memory consolidation Neurons Afferent CREB1 Cyclic AMP Response Element-Binding Protein Brief Communications Neuroscience Transcription factor |
| Zdroj: | The Journal of neuroscience : the official journal of the Society for Neuroscience. 28(8) |
| ISSN: | 1529-2401 |
| Popis: | The transcription factor cAMP response element (CRE)-binding protein (CREB) plays an essential role in the induction of many forms of long-term synaptic plasticity. Levels of CREB1, theAplysiahomolog of CREB, show sustained elevations for several hours after the induction of long-term synaptic facilitation (LTF). Furthermore, CREB1 binds to the promoter of its own gene. These results suggest the existence of a CREB1-positive feedback loop that contributes to the consolidation of LTF. In the present study, we provide a detailed, quantitative characterization of the dynamics of CREB1 mRNA and protein as well as CREB1 phosphorylation after LTF induction. Injections of CRE oligonucleotides prevented the increase in CREB1 in response to 5-HT, corroborating the existence of the CREB1 feedback loop. This loop probably sustains CRE-dependent gene transcription, which remains elevated for at least 12 h after LTF induction. LTF is blocked by injection of CREB1 antibody after the induction phase, suggesting that the CREB1-positive feedback is required for consolidation of LTF. |
| Databáze: | OpenAIRE |
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