Initial evaluation of the use of USPIO cell labeling and noninvasive MR monitoring of human tissue-engineered vascular grafts in vivo
| Autor: | Christopher K. Breuer, Tarek M. Fahmy, Yinong Wang, Xenophon Papademetris, Jordan S. Pober, George Tellides, Tai Yi, Gregory N. Nelson, Tamar L. Mirensky, Pavel Shkarin, Erik M. Shapiro, Jason D. Roh, William Mark Saltzman |
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| Rok vydání: | 2008 |
| Předmět: |
Iron
Myocytes Smooth Muscle Contrast Media Mice SCID Biochemistry Magnetic resonance angiography Muscle Smooth Vascular Cell labeling Research Communications chemistry.chemical_compound Mice Tissue engineering In vivo Blood vessel prosthesis medicine.artery Genetics Medicine Animals Humans Magnetite Nanoparticles Molecular Biology Aorta medicine.diagnostic_test Tissue Engineering business.industry Dextrans Oxides Anatomy Ferrosoferric Oxide Blood Vessel Prosthesis Ferumoxytol chemistry Nanoparticles business Iron oxide nanoparticles Magnetic Resonance Angiography Biotechnology Biomedical engineering |
| Zdroj: | FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 22(11) |
| ISSN: | 1530-6860 |
| Popis: | This pilot study examines noninvasive MR monitoring of tissue-engineered vascular grafts (TEVGs) in vivo using cells labeled with iron oxide nanoparticles. Human aortic smooth muscle cells (hASMCs) were labeled with ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles. The labeled hASMCs, along with human aortic endothelial cells, were incorporated into eight TEVGs and were then surgically implanted as aortic interposition grafts in a C.B-17 SCID/bg mouse host. USPIO-labeled hASMCs persisted in the grafts throughout a 3 wk observation period and allowed noninvasive MR imaging of the human TEVGs for real-time, serial monitoring of hASMC retention. This study demonstrates the feasibility of applying noninvasive imaging techniques for evaluation of in vivo TEVG performance.—Nelson, G. N., Roh, J. D., Mirensky, T. L., Wang, Y., Yi, T., Tellides, G., Pober, J. S., Shkarin, P., Shapiro, E. M., Saltzman, W. M., Papademetris, X., Fahmy, T. M., Breuer, C. K.. Initial evaluation of the use of USPIO cell labeling and noninvasive MR monitoring of human tissue-engineered vascular grafts in vivo. |
| Databáze: | OpenAIRE |
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