Radioimmunoassay Screening of Dried Blood Spot Materials for Benzoylecgonine*
| Autor: | W. Harry Hannon, Randy Hanzlick, David H Vroon, Barbara B. Miller, M. Louise Martin, Mary K. Powell, William R. Sexson, L. Omar Henderson |
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| Rok vydání: | 1993 |
| Předmět: |
Health
Toxicology and Mutagenesis Urinary system Radioimmunoassay Analytical chemistry Urine Toxicology Gas Chromatography-Mass Spectrometry Analytical Chemistry chemistry.chemical_compound Cocaine medicine Humans Environmental Chemistry Whole blood Chemical Health and Safety Chromatography medicine.diagnostic_test business.industry Infant Newborn United States Dried blood spot Substance Abuse Detection chemistry Specimen collection Immunoassay Benzoylecgonine Reagent Kits Diagnostic business |
| Zdroj: | Journal of Analytical Toxicology. 17:42-47 |
| ISSN: | 1945-2403 0146-4760 |
| DOI: | 10.1093/jat/17.1.42 |
| Popis: | used as urinary screening tests followed by confirmation by GC/MS. Immunoassay tests have been used primarily on urine specimens because of the ease of specimen collection and the relative abundance of the metabolite in urine following cocaine use. Levels of BE in blood are lower than those found in urine in concurrent samples, but they remain in the detectable range of immunoassays. The predictable half-life of BE in blood for extended periods has not been calculated, and the relationship between levels in blood and urine has not been correlated. Moreover, metabolism of BE in the blood during pregnancy and its half-life in the blood of newborns have not been investigated. Previously, investigators have described several techniques for the extraction of BE from blood using organic solvent extraction and subsequent assay in an aqueous or methanolic medium (5-7). Here, the authors describe the use of a commercial urinary BE immunoassay procedure with standards, calibrators, and quality control materials (QC) made up in aqueous eluates from blood spots or BE spiked into whole blood, spotted onto filter paper, and then eluted in aqueous solution. Postmortem blood samples were quantitated as blood spots for BE, and quantitation was confirmed by GC/MS analysis. The correlation of blood and urine levels of BE was measured on samples from pregnant women. Blood samples were collected from mothers; cord and blood spots from neonates were measured for BE. Finally, the quantitative immunoassay was used to measure BE levels in more than 500 anonymous residual blood spots obtained routinely from infants born in three states, at hospitals where high prevalence of cocaine use among childbearing women is suspected. |
| Databáze: | OpenAIRE |
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