Rapid, Sensitive, Full-Genome Sequencing of Severe Acute Respiratory Syndrome Coronavirus 2

Autor:	Krista Queen, Clinton R. Paden, Suxiang Tong, Anna Uehara, Yan Li, Ying Tao, Jing Zhang
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	Microbiology (medical) Epidemiology Expedited 030231 tropical medicine Pneumonia Viral coronavirus lcsh:Medicine Genomics Biology medicine.disease_cause Genome lcsh:Infectious and parasitic diseases 03 medical and health sciences symbols.namesake Betacoronavirus respiratory infections 0302 clinical medicine Multiplex polymerase chain reaction medicine genomics Multiplex viruses lcsh:RC109-216 030212 general & internal medicine high-throughput nucleotide sequencing Rapid Sensitive Full-Genome Sequencing of Severe Acute Respiratory Syndrome Coronavirus 2 Pandemics Coronavirus Sanger sequencing Whole genome sequencing Whole Genome Sequencing Sequence Analysis RNA SARS-CoV-2 lcsh:R Dispatch COVID-19 Virology zoonoses Infectious Diseases coronavirus disease whole-genome sequencing Minion symbols RNA Viral Coronavirus Infections Multiplex Polymerase Chain Reaction severe acute respiratory syndrome coronavirus 2
Zdroj:	Emerging Infectious Diseases, Vol 26, Iss 10, Pp 2401-2405 (2020) Emerging Infectious Diseases
ISSN:	1080-6059 1080-6040
Popis:	We describe validated protocols for generating high-quality, full-length severe acute respiratory syndrome coronavirus 2 genomes from primary samples. One protocol uses multiplex reverse transcription PCR, followed by MinION or MiSeq sequencing; the other uses singleplex, nested reverse transcription PCR and Sanger sequencing. These protocols enable sensitive virus sequencing in different laboratory environments.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5e1fd5993fd93acbc506c411b0b630d1 https://wwwnc.cdc.gov/eid/article/26/10/20-1800_article Zobrazit plný text záznamu Plný text ve formátu PDF