High-intensity training induces non-stoichiometric changes in the mitochondrial proteome of human skeletal muscle without reorganisation of respiratory chain content
| Autor: | Kevin Huynh, Tegan Stait, Cesare Granata, Melinda T. Coughlan, David Bishop, Natalie A. Mellett, H. Janssen, Jujiao Kuang, Nikeisha J Caruana, Nicholas A. Jamnick, Peter J. Meikle, David A. Stroud, Boris Reljic, Adrienne Laskowski, David R. Thorburn, Javier Botella, Ann E. Frazier |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Proteomics
Adult Male Adolescent Proteome Bioenergetics Biopsy In silico Science education Respiratory chain General Physics and Astronomy Oxidative phosphorylation High-Intensity Interval Training Biology Article Oxidative Phosphorylation General Biochemistry Genetics and Molecular Biology Electron Transport Young Adult Adenosine Triphosphate medicine Humans Transcriptomics Muscle Skeletal Multidisciplinary High intensity Skeletal muscle Energy metabolism General Chemistry Adaptation Physiological Mitochondrial proteome Healthy Volunteers Mitochondria Cell biology medicine.anatomical_structure Quality of Life Electron flow Fat metabolism |
| Zdroj: | Nature Communications, Vol 12, Iss 1, Pp 1-18 (2021) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | Mitochondrial defects are implicated in multiple diseases and aging. Exercise training is an accessible, inexpensive therapeutic intervention that can improve mitochondrial bioenergetics and quality of life. By combining multiple omics techniques with biochemical and in silico normalisation, we removed the bias arising from the training-induced increase in mitochondrial content to unearth an intricate and previously undemonstrated network of differentially prioritised mitochondrial adaptations. We show that changes in hundreds of transcripts, proteins, and lipids are not stoichiometrically linked to the overall increase in mitochondrial content. Our findings suggest enhancing electron flow to oxidative phosphorylation (OXPHOS) is more important to improve ATP generation than increasing the abundance of the OXPHOS machinery, and do not support the hypothesis that training-induced supercomplex formation enhances mitochondrial bioenergetics. Our study provides an analytical approach allowing unbiased and in-depth investigations of training-induced mitochondrial adaptations, challenging our current understanding, and calling for careful reinterpretation of previous findings. Exercise training can be therapeutic but how mitochondria respond remains unclear. Here, the authors use multiple omics techniques to reveal a complex network of non-stoichiometric mitochondrial adaptations that are prioritized or deprioritised during different phases of exercise training. |
| Databáze: | OpenAIRE |
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