Immunohistochemical Studies of Cytokeratins and Differentiation Markers in Bovine Ocular Squamous Cell Carcinoma
| Autor: | C. Pinto, Lluís Ferrer, Maria C. Peleteiro, João R. Mesquita, Helena Vala, Maria Pereira, Tânia Carvalho, D. Fondevila |
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| Přispěvatelé: | Repositório da Universidade de Lisboa |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Pathology
medicine.medical_specialty Biology Article involucrin Malignant transformation Cornified envelope Cytokeratin Keratin medicine Neoplastic transformation Involucrin chemistry.chemical_classification lcsh:Veterinary medicine General Veterinary bovine profilaggrin Bovine ocular squamous cell carcinoma Immunohistochemistry chemistry Profilaggrin immunohistochemistry lcsh:SF600-1100 cytokeratin Immunostaining Ocular squamous cell carcinoma |
| Zdroj: | Veterinary Sciences Volume 7 Issue 2 Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona Veterinary Sciences, Vol 7, Iss 70, p 70 (2020) Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| Popis: | © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Bovine Ocular Squamous Cell Carcinoma is considered the most common bovine tumour, causing significant economic losses, mainly by abattoir condemnations. To obtain a better insight into the genesis and neoplastic transformation, 19 samples collected at slaughter from Holstein Friesian cattle and diagnosed as Ocular Squamous Cell Carcinoma were studied. Tumours were histologically classified into three categories: poorly (26.3%), moderately (26.3%), and well differentiated (47.4%). Expression of keratins (MNF116 and LP34) and of cornified envelope precursors (involucrin and profilaggrin) was studied. Expression of MNF116 was observed in all carcinomas. LP34 immunostaining was seen in all but three carcinomas, one from each degree. Involucrin immunoreaction was observed in all but one poorly differentiated carcinoma. Profilaggrin was present in only two moderately differentiated carcinomas, in all but one well differentiated, and in all but one poorly differentiated. MNF116 is a useful marker to confirm the epithelial origin of the tumour and stain most neoplastic cells in these tumours. The expression of involucrin and LP34 demonstrates that, in all tumours, cells have reached the final program of differentiation, regardless of the grade. The expression of profilaggrin could indicate molecular changes during malignant transformation but their expression does not seem to be of diagnostic value. This research was funded by Interdisciplinary Investigation Centre for Animal Health (CIISA) from the Faculty of Veterinary Medicine, Lisbon, and by Portuguese Foundation for Science and Technology (FCT) under the project UIDB/04033/2020. |
| Databáze: | OpenAIRE |
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