Proteomic Analysis of Peripheral Blood Mononuclear Cells: Selective Protein Processing Observed in Patients with Rheumatoid Arthritis
| Autor: | Helmut Dotzlaw, Stefan Mikkat, Gunther Neeck, Martin Schulz, Martin Eggert |
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| Rok vydání: | 2007 |
| Předmět: |
Adult
Male Proteomics Biochemistry Peripheral blood mononuclear cell Autoimmune Diseases Arthritis Rheumatoid Peptide mass fingerprinting Western blot Healthy control medicine Humans Electrophoresis Gel Two-Dimensional In patient Aged Aged 80 and over Inflammation medicine.diagnostic_test business.industry Synovial Membrane General Chemistry Middle Aged medicine.disease Gene Expression Regulation Rheumatoid arthritis Immunology Proteome Protein processing Leukocytes Mononuclear Female business |
| Zdroj: | Journal of Proteome Research. 6:3752-3759 |
| ISSN: | 1535-3907 1535-3893 |
| DOI: | 10.1021/pr070285f |
| Popis: | In a comparative proteome analysis of peripheral blood mononuclear cells (PBMCs), we analyzed 130 two-dimensional gels obtained from 33 healthy control individuals and 32 patients diagnosed with rheumatoid arthritis (RA). We found 16 protein spots that are deregulated in patients with RA and, using peptide mass fingerprinting and Western blot analyses, identified these spots as belonging to 9 distinct proteins. A hierarchical clustering procedure organizes the study subjects into two main clusters based on the expression of these 16 protein spots, one that contains mostly healthy control individuals and the other mostly RA patients. The majority of the proteins differentially expressed in RA patients when compared with healthy controls can be detected as protein fragments in PBMCs obtained from RA patients. This set of deregulated proteins includes several factors that have been shown to be autoantigens in autoimmune diseases. |
| Databáze: | OpenAIRE |
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