Glycoprotein-based enzyme-linked immunosorbent assays for serodiagnosis of infectious laryngotracheitis
| Autor: | Haichen Song, Chinta Lamichhane, Mallikarjuna Kanabagatte Basavarajappa, Siba K. Samal |
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| Rok vydání: | 2014 |
| Předmět: |
Microbiology (medical)
Veterinary Medicine Enzyme-Linked Immunosorbent Assay Antibodies Viral Newcastle disease Virus Serology law.invention Herpesvirus 1 Gallid law Animals Serologic Tests Seroconversion Poultry Diseases chemistry.chemical_classification biology Herpesviridae Infections biology.organism_classification Virology Retraction Vaccination chemistry biology.protein Recombinant DNA Antibody Glycoprotein Chickens |
| Zdroj: | Journal of clinical microbiology. 53(5) |
| ISSN: | 1098-660X |
| Popis: | For detection of infectious laryngotracheitis virus (ILTV) antibody, glycoprotein B-, C-, and D-based enzyme-linked immunosorbent assays (B-, C-, and D-ELISAs, respectively) were developed. The B- and D-ELISAs showed enhanced detection of anti-ILTV antibodies in infected chickens compared to that of the commercial ELISA. Furthermore, the D-ELISA was efficient in detecting seroconversion with vectored vaccine, using recombinant Newcastle disease virus (rNDV) expressing glycoprotein D (gD) as the vaccine vector. |
| Databáze: | OpenAIRE |
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