Bone formation in a rat calvarial defect model after transplanting autogenous bone marrow with beta-tricalcium phosphate
| Autor: | Tatsushi Matsumura, Miwa Kanou, Toshimasa Kagawa, Hideto Imura, Nobuaki Shirasu, Akiko Ota, Masako Sawaki, Kazuo Sano, Yasuhisa Hirata, Takaaki Ueno, Mari Wakimoto, Azumi Hirata, Eiki Yamachika, Tomohiro Yamada |
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| Rok vydání: | 2010 |
| Předmět: |
Calcium Phosphates
Male medicine.medical_specialty Bone Regeneration Histology Acid Phosphatase Biocompatible Materials Bone Marrow Cells Core Binding Factor Alpha 1 Subunit Calvaria Tissue engineering Osteogenesis Internal medicine medicine Animals Osteopontin Tibia Bone regeneration Bone Marrow Transplantation Cell Proliferation Tartrate-resistant acid phosphatase Wound Healing Tissue Engineering biology Tartrate-Resistant Acid Phosphatase Chemistry Skull Cell Differentiation Cell Biology General Medicine Rats Surgery Isoenzymes Disease Models Animal medicine.anatomical_structure Endocrinology Bone Substitutes biology.protein Bone marrow |
| Zdroj: | Acta Histochemica. 112:270-277 |
| ISSN: | 0065-1281 |
| Popis: | In the present study, we evaluated the osteogenic potential of an autogenous bone marrow graft combined with beta-tricalcium phosphate (beta-TCP) in a rat calvarial bone defect model. The bone marrow harvested from the tibia of 7-week-old rats was grafted autogenously in a calvarial defect together with beta-TCP (=BTG group, n=16) or without beta-TCP (=BG group, n=16). Groups of animals were also treated with beta-TCP alone (=TG group, n=16) and control animals (n=8) received no graft implanted into the defect. We then observed the process of bone formation by histology, enzyme histochemistry and immunohistochemistry. Five days after grafting, in the BTG and BG groups, cell proliferation and osteogenic differentiation were observed. From 5 to 10 days after surgery, active Runx2, osteopontin (OPN), and TRAP- positive cells appeared in the BTG and BG groups. New bone formation started in the defect in both the BTG and BG groups. At 30 days after grafting, the BTG group showed new bone development and replacement of beta-TCP to fill the bone defect. New bone formation in the BTG group was significantly greater than in the BG group (P |
| Databáze: | OpenAIRE |
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