Proteome Analysis of Responses to Ascochlorin in a Human Osteosarcoma Cell Line by 2-D Gel Electrophoresis and MALDI-TOF MS
| Autor: | Kunio Ando, Jeong Han Kang, In-Seon Lee, Young-Chae Chang, Cheorl-Ho Kim, Kwan-Kyu Park, Junji Magae |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Proteomics
MAPK/ERK pathway Proteome Blotting Western Antineoplastic Agents Bone Neoplasms Alkenes Biology Biochemistry Phenols Minichromosome maintenance Cell Line Tumor Biomarkers Tumor Humans Electrophoresis Gel Two-Dimensional Zymography Osteosarcoma Reverse Transcriptase Polymerase Chain Reaction General Chemistry Molecular biology Neoplasm Proteins Blot Matrix-assisted laser desorption/ionization Cell culture Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization |
| Zdroj: | Journal of Proteome Research. 5:2620-2631 |
| ISSN: | 1535-3907 1535-3893 |
| DOI: | 10.1021/pr060111i |
| Popis: | Ascochlorin is a prenyl-phenol compound that was isolated from the fungus Ascochyta viciae. Ascochlorin reduces serum cholesterol and triglyceride levels, suppresses hypertension and tumor development, and ameliorates type I and II diabetes. Here, to better understand the mechanisms by which ascochlorin regulates physiological or pathological events and induces responses in the pharmacological treatment of cancer, we performed differential analysis of the proteome of the human osteosarcoma cells U2OS in response to ascochlorin. In addition, we established the first two-dimensional map of the U2OS proteome. The U2OS cell proteomes with and without treatment with ascochlorin were compared using two-dimensional electrophoresis, matrix-assisted laser desorption/ionization mass spectrometry and bioinformatics. The largest differences in expression were observed for the epidermal growth factor receptor (4-fold decrease), ribulose-5-phosphate-epimerase (13-fold decrease), ATP-dependent RNA helicase (8-fold decrease), and kelch-like ECH-associated protein 1 (6-fold decrease). The abundance of heterogeneous nuclear ribonucleoprotein L and minichromosome maintenance protein 7 increased 12- and 8.2-fold, respectively. In addition, Erk 2 was increased 3-fold in U2OS cells treated with ascochlorin. The expression of some selected proteins was confirmed by western blotting, zymography and RT-PCR analysis. |
| Databáze: | OpenAIRE |
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