Changes in Gene Expression of Arabidopsis Thaliana Cell Cultures Upon Exposure to Real and Simulated Partial-g Forces
| Autor: | Margret Ecke, Rüdiger Hampp, Jens Hauslage, Maren Neef, Ina Spirer, Svenja Fengler |
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| Rok vydání: | 2015 |
| Předmět: |
0106 biological sciences
0301 basic medicine Arabidopsis thaliana General Physics and Astronomy 01 natural sciences Partial-g forces 03 medical and health sciences Arabidopsis Gene expression Parabolic flight Gene biology Chemistry Microarray analysis techniques Applied Mathematics General Engineering Cell cultures biology.organism_classification Cell biology 030104 developmental biology Cell culture Modeling and Simulation Nucleic acid Microgravity Clinostat 010606 plant biology & botany |
| Zdroj: | Microgravity Science and Technology. 28:319-329 |
| ISSN: | 1875-0494 0938-0108 |
| DOI: | 10.1007/s12217-015-9452-y |
| Popis: | Cell cultures of the plant model organism Arabidopsis thaliana were exposed to partial-g forces during parabolic flight and clinostat experiments (0.16 g, 0.38 g and 0.5 g were tested). In order to investigate gravity-dependent alterations in gene expression, samples were metabolically quenched by the fixative RNAlater Ⓡ to stabilize nucleic acids and used for whole-genome microarray analysis. An attempt to identify the potential threshold acceleration for the gravity-dependent response showed that the smaller the experienced g-force, the greater was the susceptibility of the cell cultures. Compared to short-term μ g during a parabolic flight, the number of differentially expressed genes under partial-g was lower. In addition, the effect on the alteration of amounts of transcripts decreased during partial-g parabolic flight due to the sequence of the different parabolas (0.38 g, 0.16 g and μ g). A time-dependent analysis under simulated 0.5 g indicates that adaptation occurs within minutes. Differentially expressed genes (at least 2-fold up- or down-regulated in expression) under real flight conditions were to some extent identical with those affected by clinorotation. The highest number of homologuous genes was detected within seconds of exposure to 0.38 g (both flight and clinorotation). To a considerable part, these genes deal with cell wall properties. Additionally, responses specific for clinorotation were observed. |
| Databáze: | OpenAIRE |
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