Survey of major genotypes and subtypes of hepatitis C virus using RFLP of sequences amplified from the 5' non-coding region
| Autor: | F. Davidson, P. Simmonds, J. C. Ferguson, L. M. Jarvis, B. C. Dow, E. A. C. Follett, C. R. G. Seed, T. Krusius, C. Lin, G. A. Medgyesi, H. Kiyokawa, G. Olim, G. Duraisamy, T. Cuypers, A. A. Saeed, D. Teo, J. Conradie, M. C. Kew, M. Lin, C. Nuchaprayoon, O. K. Ndimbie, P. L. Yap |
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| Rok vydání: | 1995 |
| Předmět: |
Genetics
biology Genotype Hepatitis C virus Hepacivirus Hcv transmission Blood Donors medicine.disease_cause biology.organism_classification Virology Polymerase Chain Reaction law.invention Restriction enzyme law medicine Coding region Humans Restriction fragment length polymorphism Polymerase chain reaction Polymorphism Restriction Fragment Length |
| Zdroj: | The Journal of general virology. 76 |
| ISSN: | 0022-1317 |
| Popis: | A method is described for identifying different genotypes of hepatitis C virus (HCV) by restriction endonuclease cleavage of sequences amplified by PCR from the 5′ non-coding region. Using the enzymes HaeIII-RsaI and HinfI-MvaI, followed by cleavage with BstU1 or ScrFI, it was possible to identify and distinguish HCV genotypes 1a, 1b, 2a, 2b, 3a, 3b, 4, 5 and 6. The method was used to investigate the prevalence of these genotypes in 723 blood donors in 15 countries, the largest survey to date, and one which covered a wide range of geographical regions (Europe, America, Africa and Asia). These results, combined with a review of the existing literature, indicate the existence of several distinct regional patterns of HCV genotype distribution, and provide the framework for future detailed epidemiological investigations of HCV transmission. |
| Databáze: | OpenAIRE |
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