SOX9 gene plus heparinized TGF-β 3 coated dexamethasone loaded PLGA microspheres for inducement of chondrogenesis of hMSCs
| Autor: | Ji Sun Park, Keun-Hong Park, Dae Gyun Woo, Su Yeon Jeon, Han Na Yang |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Time Factors Materials science Cell Survival Polymers Biophysics Mice Nude Tetrazolium Salts Bioengineering Dexamethasone Biomaterials Extracellular matrix Glycosaminoglycan Mice Chondrocytes Transforming Growth Factor beta3 Polylactic Acid-Polyglycolic Acid Copolymer Tissue engineering In vivo Cell Adhesion Animals Humans Lactic Acid Aggrecan Glycosaminoglycans Mice Inbred BALB C Heparin Regeneration (biology) Mesenchymal Stem Cells SOX9 Transcription Factor Chondrogenesis Molecular biology Microspheres In vitro Thiazoles Gene Expression Regulation Mechanics of Materials Ceramics and Composites Female Polyglycolic Acid Biomedical engineering |
| Zdroj: | Biomaterials. 33:7151-7163 |
| ISSN: | 0142-9612 |
| DOI: | 10.1016/j.biomaterials.2012.06.023 |
| Popis: | Microparticulated types of scaffolds have been widely applied in stem cell therapy and the tissue engineering field for the regeneration of wound tissues. During application of simple genes or growth factors and cell delivery vehicles, we designed a method that employs dexamethsone loaded PLGA microspheres consisting of polyplexed SOX9 genes plus heparinized TGF-β 3 on the surface of polymeric microspheres prepared using a layer-by-layer (LbL) method. The fabrication of the polyplexed SOX9 genes plus heparinized TGF-β 3 and their subsequent coating onto dexamethsone loaded PLGA microspheres represents a method for functionalization of the polymeric matrix. The use of SOX9 gene plus heparinized TGF-β 3 coated dexamethsone loaded PLGA microspheres was evaluated to determine their potential as both gene carriers and cell delivery vehicle. By adhesion of hMSCs onto SOX9 gene plus heparinized TGF-β 3 coated dexamethsone loaded PLGA microspheres, the chondrogenesis-related specific genes of collagen type II were increased 30 times comparing to control. Also, the specific extracellular matrix of glycosaminoglycan (GAG) production of hMSCs adhered onto SOX9 gene plus heparinized TGF-β 3 coated dexamethasone loaded PLGA microspheres increased more 2.5 times than control group. Not only in vitro culture but in vivo results, the specific genes of COMP, aggrecan, collagen type II, and SOX9 showed much more gene expressions such as 20, 15, 10, 8 times. |
| Databáze: | OpenAIRE |
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