Very low levels of surface CD45 reflect CLL cell fragility, are inversely correlated with trisomy 12 and are associated with increased treatment-free survival

Autor: Franck Trimoreau, Ibtissam Marfak, Arnaud Jaccard, David Rizzo, Jean Feuillard, Nathalie Gachard, Estelle Guérin, Dominique Bordessoule, Jean-Luc Faucher, Angad Lotay
Přispěvatelé: Contrôle de la Réponse Immune B et des Lymphoproliférations (CRIBL), Université de Limoges (UNILIM)-Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST FR CNRS 3503)-Centre National de la Recherche Scientifique (CNRS), Service d'Hématologie biologique [CHU Limoges], CHU Limoges, Service d'Hématologie clinique et thérapie cellulaire [CHU Limoges]
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Chronic lymphocytic leukemia
Cell
MESH: Membrane Glycoproteins
Gene Expression
Trisomy
MESH: Down-Regulation
0302 clinical medicine
MESH: Lymphoma
Non-Hodgkin
immune system diseases
hemic and lymphatic diseases
Gene expression
MESH: Leukemia
Lymphocytic
Chronic
B-Cell
B-Lymphocytes
0303 health sciences
Membrane Glycoproteins
Lymphoma
Non-Hodgkin
Hematology
MESH: Neoplasm Staging
MESH: Case-Control Studies
Leukemia
medicine.anatomical_structure
030220 oncology & carcinogenesis
Disease Progression
MESH: Trisomy
[SDV.IMM]Life Sciences [q-bio]/Immunology
MESH: Disease Progression
MESH: Gene Expression
Down-Regulation
MESH: Antigens
CD45
Biology
Disease-Free Survival
03 medical and health sciences
Downregulation and upregulation
Osmotic Pressure
MESH: B-Lymphocytes
medicine
Humans
Neoplasm Staging
030304 developmental biology
MESH: Humans
MESH: Antigens
CD38
Case-control study
MESH: Biological Markers
MESH: Osmotic Pressure
medicine.disease
ADP-ribosyl Cyclase 1
Leukemia
Lymphocytic
Chronic
B-Cell
Lymphoma
Case-Control Studies
Immunology
MESH: Disease-Free Survival
Leukocyte Common Antigens
Biomarkers
Zdroj: American Journal of Hematology
American Journal of Hematology, Wiley, 2013, 88 (9), pp.747-53. ⟨10.1002/ajh.23494⟩
ISSN: 0361-8609
1096-8652
DOI: 10.1002/ajh.23494⟩
Popis: International audience; It has recently been suggested that the percentage of smudge cells on blood smears from patients with chronic lymphocytic leukemia (CLL) could predict overall survival. However, smudge cells are a cytological artifact influenced by multiple physical factors not related to CLL. To identify simple parameters reflecting CLL cell fragility, we studied CD45 expression in a series of 66 patients with Binet stage A CLL. Decreased CD45 expression was specific for CLL cells when compared to 44 patients with a leukemic phase of B-cell non Hodgkin lymphoma and 42 control B-cells. CD45 expression was markedly decreased for all patients with CLL with high percentages of smudge cells. CLL cells with the lowest CD45 expression were the most sensitive to osmotic shock. Very low levels of CD45 expression were significantly associated with lack of CD38 expression, absence of trisomy 12, and with increased treatment free survival time. Altogether, these results demonstrate that low levels of CD45 expression are specific to CLL cells and reflect cell fragility, suggesting that this is an important intrinsic biological feature that determines disease course.
Databáze: OpenAIRE
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