LncRNA MALAT1 promotes wound healing via regulating miR-141-3p/ZNF217 axis

Autor: Yun-Chuan Pan, Zun-Hong Liang, Zhi Zhang, Zhi-Yang Qiu, Shi-Shuai Lin
Rok vydání: 2020
Předmět:
0301 basic medicine
PVDF
polyvinylidene fluoride
ZNF217
zinc-finger protein 217
Biomedical Engineering
Wound healing
ZEB1
E-box binding homeobox 1
EMT
epithelial mesenchymal transition
Matrix (biology)
TGF-β2
Transforming Growth Factor-β2
lncRNA
long non-coding RNA
Biomaterials
03 medical and health sciences
0302 clinical medicine
ELISA
enzyme linked immunosorbent assay
medicine
ZNF217
lcsh:QH573-671
MALAT1
Fibroblast
MALAT1
metastasis-associated lung adenocarcinoma transcript 1
miR-141-3p
lcsh:R5-920
Gene knockdown
Viral matrix protein
lcsh:Cytology
Chemistry
SDS-PAGE
sodium dodecyl sulfate-polyacrylamide gel electrophoresis
qRT-PCR
quantitative real-time PCR
ECM
extra cellular matrix
Cell biology
Blot
030104 developmental biology
medicine.anatomical_structure
Original Article
Signal transduction
lcsh:Medicine (General)
MTT
3-(4
5-dimethyl-2-thiazolyl)-2
5-diphenyl-2-H-tetrazolium bromide
HFF-1
human fibroblast cells
030217 neurology & neurosurgery
Developmental Biology
Zdroj: Regenerative Therapy, Vol 15, Iss, Pp 202-209 (2020)
Regenerative Therapy
ISSN: 2352-3204
DOI: 10.1016/j.reth.2020.09.006
Popis: Background The process of wound healing is complex. Increasing evidences have shown that lncRNA MALAT1 is abundant in fibroblasts and may be engaged in wound healing process. Therefore, we explored the mechanism of MALAT1 affecting wound healing. Methods The expression levels of MALAT1, miR-141-3p as well as ZNF217 in human fibroblast cells (HFF-1) were quantified by qRT-PCR. HFF-1 proliferation was measured by MTT, while migration was detected by wound healing assay. SMAD2 activation and matrix proteins expression were detected by western blotting. The interaction between miR-141-3p and MALAT1 or ZNF217 was further confirmed using the luciferase reporter gene assay. In vivo wound healing was assessed by full-thickness wound healing model on C57BL/6 mice. Result Knockdown of MALAT1 as well as overexpression miR-141-3p remarkably inhibited the proliferation, migration and matrix protein expression in HFF-1 cells. MALAT1 directly targeted and inhibited the expression of miR-141-3p. MiR-141-3p suppressed the activation of TGF-β2/SMAD2 signaling pathway by targeting ZNF217. Knockdown of MALAT1 inhibited wound healing process in mice. Conclusions MALAT1 up-regulates ZNF217 expression by targeting miR-141-3p, thus enhances the activity of TGF-β2/SMAD2 signaling pathway and promotes wound healing process. This investigation shed new light on the understanding of the role of MALAT1 in wound healing, and may provide potential target for the diagnosis or therapy of chronic wounds.
Databáze: OpenAIRE
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